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Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides
Zhou, Jianqiao1; Liang, Yulu1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3
KeywordPhosphopeptide Enrichment Magnetic Nanomaterials Metal-organic Framework Immobilized Metal Ion Affinity Chromatography Metal Oxide Affinity Chromatography
Source PublicationACS SUSTAINABLE CHEMISTRY & ENGINEERING
2017-12-01
ISSN2168-0485
DOI10.1021/acssuschemeng.7b02521
Volume5Issue:12Pages:11413-11421
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Multidisciplinary ; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY ; Engineering, Chemical
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Engineering
WOS KeywordMALDI-TOF MS ; ION AFFINITY-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; SELECTIVE ENRICHMENT ; EFFICIENT ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PROTEIN-PHOSPHORYLATION ; BASIC PHOSPHOPEPTIDES ; FACILE PREPARATION ; NANOPARTICLES
AbstractThe highly specific enrichment of phosphoproteins and phosphopeptides from intricate biological systems is the precondition of in-depth phosphoproteome research. Herein, a novel dual-functionalized magnetic zirconium-based metal-organic framework (MOF) denoted as DFMMOF, with the purpose of combining the affinity of immobilized metal ion affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC) has been successfully synthesized. The inherent Zr-O cluster of DFMMOF particles acted as MOAC and the immobilized titanium(IV) ions served for IMAC. The obtained DFMMOF exhibited rapid magnetic separation (within 5 s), large surface area (237.9 m(2) g(-1)), high binding capacity (100 mg g(-1)), and good postenrichment recovery (84.8%). Thanks to the strong affinity, low detection sensitivity (5 fmol) and high selectivity (beta-casein/BSA with a molar ratio of 1:1000) for phosphopeptide enrichment were obtained using DFMMOF as absorbent. Moreover, the effective identification of phosphopeptides from real samples (human serum and nonfat milk) further confirmed the immense potential of DFMMOF as a promising candidate for the detection and extraction of trace amounts of phosphorylated peptides in complex biosamples.
Language英语
WOS IDWOS:000417341900032
PublisherAMER CHEMICAL SOC
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Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169223
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen, Langxing
Affiliation1.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Coll Chem, Tianjin 300071, Peoples R China
2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Jianqiao,Liang, Yulu,He, Xiwen,et al. Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2017,5(12):11413-11421.
APA Zhou, Jianqiao,Liang, Yulu,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,5(12),11413-11421.
MLA Zhou, Jianqiao,et al."Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 5.12(2017):11413-11421.
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