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Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry
Wang, Shuangyuan1,2; Zhou, Lina1; Wang, Zhichao1,2; Shi, Xianzhe1; Xu, Guowang1
KeywordMetabolomics Lipidomics Metabolite Coverage Two-dimensional Liquid Chromatography Heart-cutting
Source PublicationANALYTICA CHIMICA ACTA
2017-05-08
ISSN0003-2670
DOI10.1016/j.aca.2017.03.004
Volume966Pages:34-40
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTARGETED METABOLOMICS ; REVERSED-PHASE ; UPLC-MS ; COVERAGE ; SEPARATION ; PLASMA ; IDENTIFICATION ; METABOLISM ; DISEASE ; CANCER
AbstractIncreasing metabolite coverage by combining data from different platforms or methods can improve understanding of related metabolic mechanisms and the identification of biomarkers. However, no one method can obtain metabolomic and lipidomic information in a single analysis. In this work, aiming at collecting comprehensive information on metabolome and lipidome in a single analytical run, we developed an on-line heart-cutting two-dimensional liquid chromatography-mass spectrometry (2D-LCMS) method. Complex metabolites from biological samples are divided into two fractions by using a precolumn. The first fraction is directly transferred and subjected to metabolomics analysis. Most lipids are retained on the precolumn until the mobile phases for lipidomics flow through; then they are subjected to lipidomics analysis. Up to 447 and 289 metabolites in plasma, including amino acids, carnitines, bile acids, free fatty acids, lyso-phospholipids, phospholipids, sphingomyelins etc. were identified within 30 min in the positive mode and negative mode, respectively. A comparison of the newly developed method with the conventional metabolomic and lipidomic approaches showed that approximately 99% features obtained by the two conventional methods can be covered with this 2D-LC method. Analytical characteristics evaluation showed the method had a wide linearity range, high sensitivity, satisfactory recovery and repeatability. These results demonstrate that this method is reliable, stable and well qualified in metabolomics analysis, particularly for large-scale metabolomics studies with small amount of samples. (C) 2017 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000398435500003
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169313
Collection中国科学院大连化学物理研究所
Corresponding AuthorShi, Xianzhe; Xu, Guowang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shuangyuan,Zhou, Lina,Wang, Zhichao,et al. Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2017,966:34-40.
APA Wang, Shuangyuan,Zhou, Lina,Wang, Zhichao,Shi, Xianzhe,&Xu, Guowang.(2017).Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry.ANALYTICA CHIMICA ACTA,966,34-40.
MLA Wang, Shuangyuan,et al."Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry".ANALYTICA CHIMICA ACTA 966(2017):34-40.
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