DICP OpenIR
Synthetic Cofactor-Linked Metabolic Circuits for Selective Energy Transfer
Wang, Lei1,2,3; Ji, Debin1; Liu, Yuxue1; Wang, Qian1,2; Wang, Xueying1; Zhou, Yongjin J.1; Zhang, Yixin1; Liu, Wujun1,2; Zhao, Zongbao K.1,2,4
KeywordEnergy Metabolism Non-natural Redox Cofactor Metabolic Circuit Synthetic Biology Nicotinamide Cytosine Dinucleotide Phosphite Dehydrogenase
Source PublicationACS CATALYSIS
2017-03-01
ISSN2155-5435
DOI10.1021/acscata1.6b03579
Volume7Issue:3Pages:1977-1983
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Physical
WOS Research AreaChemistry
WOS KeywordESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; L-MALATE ; SYSTEM ; ENZYME ; ACID ; MUTANT ; NADH
AbstractA cellular energy-transfer process can be analogized as the running of an energy carrier (EC)-linked metabolic circuit between an energy supplying module (ESM) and an energy-utilizing module (EUM). Because natural EC such as the reduced nicotinamide adenosine dinucleotide (NAD) links multiple energy-transfer modules and metabolic circuits, and the formation of natural EC is routinely coupled with the transformation of endogenous substances, it is challenging to transfer energy selectively. Here we devise synthetic cofactor -linked circuits for pathway-selective energy transfer. We engineer phosphite dehydrogenase as ESM to use the synthetic cofactor nicotinamide cytosine dinucleotide (NCD). We construct diverse circuits in vitro by combining different ESM, EUM, and EC, and we demonstrate that an energy-transfer process is controllable by tuning the feature of each component of the circuit. More specifically, we show that it is possible to drive the NCD-linked subsystem while leaving the NAD-linked reaction virtually unaffected. When armed with such circuits, Escherichia coli cells used phosphite as the electron source to generate reduced NCD that drove reductive carboxylation of pyruvate for improved malate production from glucose. Together, this study provides an opportunity to establish an orthogonal energy-transfer system for engineering cell factories and may be used to set an additional layer of a control mechanism for life.
Language英语
WOS IDWOS:000395726500058
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169353
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Wujun; Zhao, Zongbao K.
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
3.Northeast Elect Power Univ, Coll Chem Engn, Inst Green Convers Biol Bioresource & Metab Engn, Beijing 132012, Jilin, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Lei,Ji, Debin,Liu, Yuxue,et al. Synthetic Cofactor-Linked Metabolic Circuits for Selective Energy Transfer[J]. ACS CATALYSIS,2017,7(3):1977-1983.
APA Wang, Lei.,Ji, Debin.,Liu, Yuxue.,Wang, Qian.,Wang, Xueying.,...&Zhao, Zongbao K..(2017).Synthetic Cofactor-Linked Metabolic Circuits for Selective Energy Transfer.ACS CATALYSIS,7(3),1977-1983.
MLA Wang, Lei,et al."Synthetic Cofactor-Linked Metabolic Circuits for Selective Energy Transfer".ACS CATALYSIS 7.3(2017):1977-1983.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Lei]'s Articles
[Ji, Debin]'s Articles
[Liu, Yuxue]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Lei]'s Articles
[Ji, Debin]'s Articles
[Liu, Yuxue]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Lei]'s Articles
[Ji, Debin]'s Articles
[Liu, Yuxue]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.