DICP OpenIR
NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing
Zhang, Shen1,2,3; Shan, Yichu1; Zhang, Shurong1; Sui, Zhigang1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1
KeywordDe Novo Sequencing Non-isobaric Peptide Termini Labeling Quantitative Proteomics Mass-defect Based Labeling Peptide Mutation
Source PublicationJOURNAL OF PROTEOMICS
2017-02-10
ISSN1874-3919
DOI10.1016/j.jprot.2016.12.003
Volume154Pages:40-48
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordTANDEM MASS-SPECTROMETRY ; PROTEOME QUANTIFICATION ; QUANTITATIVE PROTEOMICS ; IDENTIFICATION ; ACQUISITION ; ALGORITHM ; DIGESTION ; ION ; HCC ; MS
AbstractA simple and effective de novo sequencing strategy assisted by non-isobaric peptide termini labeling, NIPTL-Novo, was established. The y-series ions and b-series ions of peptides can be clearly distinguished according to the different mass tags incorporated in N-terminus and C-terminus. This is helpful for improving the accuracy of peptide sequencing and increasing the sequencing speed. For the spectra commonly identified by both de novo sequencing and database searching software (Mascot or Maxquant), NIPTL-Novo gave identical result to more than 85% of these spectra. Furthermore, the quantitative profiling of the sample can be performed simultaneously along with de novo sequencing. Finally, this strategy can be applied to discover the peptides with potential mutation sites by combining with mass-defect based isotopic labeling.
Language英语
WOS IDWOS:000394191200005
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169381
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, Toronto, ON, Canada
Recommended Citation
GB/T 7714
Zhang, Shen,Shan, Yichu,Zhang, Shurong,et al. NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing[J]. JOURNAL OF PROTEOMICS,2017,154:40-48.
APA Zhang, Shen.,Shan, Yichu.,Zhang, Shurong.,Sui, Zhigang.,Zhang, Lihua.,...&Zhang, Yukui.(2017).NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing.JOURNAL OF PROTEOMICS,154,40-48.
MLA Zhang, Shen,et al."NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing".JOURNAL OF PROTEOMICS 154(2017):40-48.
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