DICP OpenIR
Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus
Chiang, Cheng-Kang1,2; Xu, Bo1; Mehta, Neel3; Mayne, Janice1; Sun, Warren Y. L.1; Cheng, Kai1; Ning, Zhibin1; Dong, Jing4; Zou, Hanfa4; Cheng, Hai-Ying Mary3; Figeys, Daniel1,5,6
KeywordHippocampus Circadian Rhythm Quantitative Proteome And phosphoProteome Analysis Phosphorylation Kinase-substrate Relations
Source PublicationFRONTIERS IN NEUROLOGY
2017-03-22
ISSN1664-2295
DOI10.3389/fneur.2017.00110
Volume8
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectClinical Neurology ; Neurosciences
WOS Research AreaNeurosciences & Neurology
WOS KeywordCAMP-RESPONSIVE ELEMENT ; MEMORY-PERSISTENCE ; MOUSE-LIVER ; PROTEIN ; PHOSPHORYLATION ; KINASE ; NETWORKS ; RHYTHMS ; CORTICOSTERONE ; OSCILLATION
AbstractThe circadian clock is an endogenous oscillator that drives daily rhythms in physiology, behavior, and gene expression. The underlying mechanisms of circadian timekeeping are cell-autonomous and involve oscillatory expression of core clock genes that is driven by interconnecting transcription-translation feedback loops (TTFLs). Circadian clock TTFLs are further regulated by posttranslational modifications, in particular, phosphorylation. The hippocampus plays an important role in spatial memory and the conversion of short-to long-term memory. Several studies have reported the presence of a peripheral oscillator in the hippocampus and have highlighted the importance of circadian regulation in memory formation. Given the general importance of phosphorylation in circadian clock regulation, we performed global quantitative proteome and phosphoproteome analyses of the murine hippocampus across the circadian cycle, applying spiked-in labeled reference and high accuracy mass spectrometry (MS). Of the 3,052 proteins and 2,868 phosphosites on 1,368 proteins that were accurately quantified, 1.7% of proteins and 5.2% of phosphorylation events exhibited time-of-day-dependent expression profiles. The majority of circadian phosphopeptides displayed abrupt fluctuations at mid-to-late day without underlying rhythms of protein abundance. Bioinformatic analysis of cyclic phosphorylation events revealed their diverse distribution in different biological pathways, most notably, cytoskeletal organization and neuronal morphogenesis. This study provides the first large-scale, quantitative MS analysis of the circadian phosphoproteome and proteome of the murine hippocampus and highlights the significance of rhythmic regulation at the posttranslational level in this peripheral oscillator. In addition to providing molecular insights into the hippocampal circadian clock, our results will assist in the understanding of genetic factors that underlie rhythms-associated pathological states of the hippocampus.
Language英语
WOS IDWOS:000396978200001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169423
Collection中国科学院大连化学物理研究所
Corresponding AuthorCheng, Hai-Ying Mary; Figeys, Daniel
Affiliation1.Univ Ottawa, Ottawa Inst Syst Biol, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON, Canada
2.Natl Dong Hwa Univ, Dept Chem, Hualien, Taiwan
3.Univ Toronto, Dept Biol, Mississauga, ON, Canada
4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
5.Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON, Canada
6.Canadian Inst Adv Res, Toronto, ON, Canada
Recommended Citation
GB/T 7714
Chiang, Cheng-Kang,Xu, Bo,Mehta, Neel,et al. Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus[J]. FRONTIERS IN NEUROLOGY,2017,8.
APA Chiang, Cheng-Kang.,Xu, Bo.,Mehta, Neel.,Mayne, Janice.,Sun, Warren Y. L..,...&Figeys, Daniel.(2017).Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus.FRONTIERS IN NEUROLOGY,8.
MLA Chiang, Cheng-Kang,et al."Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus".FRONTIERS IN NEUROLOGY 8(2017).
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