DICP OpenIR
A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging
Chang, Lifang1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3
Source PublicationNANOSCALE
2017
ISSN2040-3364
DOI10.1039/c6nr09944k
Volume9Issue:11Pages:3881-3888
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS KeywordUP-CONVERSION NANOPARTICLES ; QUANTUM DOTS ; GRAPHENE OXIDE ; SEMICONDUCTOR NANOCRYSTALS ; FRET ; INHIBITORS ; AFFINITY ; PROBES ; BIOMOLECULES ; ARCHITECTURE
AbstractA novel fluorescent turn-on biosensor based on fluorescence resonance energy transfer (FRET) from GSH functionalized Mn-doped ZnS QDs to graphene oxide (GO) was constructed to determine glutathione S-transferases (GSTs) in live cells and human urine. The QDs@GSH is adsorbed on the GO surface via hydrogen bonding interaction between the GSH on the surface of QDs@GSH and GO, and as a result, fluorescence quenching of the QDs@GSH takes place because of FRET. The FRET efficiency from QDs@GSH to GO was calculated to be 86.3%. However, in the presence of GSTs, the FRET process could be inhibited by the specific interaction between the GSH on the surface of QDs@GSH and GSTs, which would keep the QDs@GSH far away from the GO surface, leading to the recovery of the fluorescence. The proposed sensor exhibited high sensitivity, selectivity, and excellent specificity in the buffer, live cells and human urine for the detection of GSTs. Under the physiological conditions (pH 7.4), dissociation constants and the detection limit of GST and ATP6 V1F (a GST-tagged protein) were estimated to be 8.0 x 10(-9) M, 2.1 x 10(-10) M and 3.5 x 10(-9) M, 7.2 x 10(-11) M, respectively. The presented method has been successfully utilized for the determination of the GSTs in live cells and human urine without any complicated pretreatment and the recovery was in the range of 80%-90%.
Language英语
WOS IDWOS:000397126000022
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169559
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen, Langxing
Affiliation1.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Chang, Lifang,He, Xiwen,Chen, Langxing,et al. A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging[J]. NANOSCALE,2017,9(11):3881-3888.
APA Chang, Lifang,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging.NANOSCALE,9(11),3881-3888.
MLA Chang, Lifang,et al."A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging".NANOSCALE 9.11(2017):3881-3888.
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