DICP OpenIR
Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study
Cong, Yuting1,2; Hu, Lianghai1; Zhang, Zhang2; Gao, Yin1; Dong, Mingming2; Qin, Hongqiang2; Ye, Mingliang2; Gu, Jingkai1; Zou, Hanfa2
KeywordProteomics Mass Spectrometry Therapeutic Monoclonal Antibody Glycosylation Parallel Reaction Monitoring Pharmacokinetics
Source PublicationTALANTA
2017-04-01
ISSN0039-9140
DOI10.1016/j.talanta.2017.01.023
Volume165Pages:664-670
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordIMMUNOGLOBULIN-G ; TARGETED PROTEOMICS ; HIGH-RESOLUTION ; HUMAN SERUM ; FC ; GLYCOPEPTIDES ; ACCURATE ; GLYCANS
AbstractMonoclonal antibodies (mAbs), are one of the most important protein drugs have attracted increasing attention. However, the pharmacokinetics of mAbs has not been fully investigated due to the complexity of protein drugs. Traditonal immuno-based approaches can not recognize the proteoforms of mAbs because of the long development cycles, prohibitive cost, and interactions between different proteins. Therefore, reliable qualitative and quantitative analysis of the proteoforms of mAbs in biological samples is of crucial importance. Herein, a novel method was developed for absolute quantitation of mAbs and their glycoforms in complex biological samples such as serum and tissues. With the combination of HILIC enrichment and parallel reaction monitoring by high resolution mass spectrometry, most of the glycoforms can be accurately quantified at the fmol level through the use of the model mAb of bevacizumab. More importantly, the structural confirmation can be achieved simultaneously without the need for additional experiments. This strategy can be readily applied to the pharmacokinetic study of glycosylation modification and biomarker discovery for clinical applications.
Language英语
WOS IDWOS:000394064700094
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169567
Collection中国科学院大连化学物理研究所
Corresponding AuthorHu, Lianghai; Ye, Mingliang
Affiliation1.Jilin Univ, Sch Life Sci, Changchun 130012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Cong, Yuting,Hu, Lianghai,Zhang, Zhang,et al. Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study[J]. TALANTA,2017,165:664-670.
APA Cong, Yuting.,Hu, Lianghai.,Zhang, Zhang.,Gao, Yin.,Dong, Mingming.,...&Zou, Hanfa.(2017).Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study.TALANTA,165,664-670.
MLA Cong, Yuting,et al."Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study".TALANTA 165(2017):664-670.
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