Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study

doi:10.1016/j.talanta.2017.01.023

DICP OpenIR

	Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study
	Cong, Yuting 1,2; Hu, Lianghai 1; Zhang, Zhang 2; Gao, Yin 1; Dong, Mingming 2; Qin, Hongqiang 2; Ye, Mingliang 2; Gu, Jingkai 1; Zou, Hanfa 2
Keyword	Proteomics Mass Spectrometry Therapeutic Monoclonal Antibody Glycosylation Parallel Reaction Monitoring Pharmacokinetics
Source Publication	TALANTA
	2017-04-01
ISSN	0039-9140
DOI	10.1016/j.talanta.2017.01.023
Volume	165 Pages:664-670
Indexed By	SCI
Subtype	Article
WOS Headings	Science & Technology ; Physical Sciences
WOS Subject	Chemistry, Analytical
WOS Research Area	Chemistry
WOS Keyword	IMMUNOGLOBULIN-G ; TARGETED PROTEOMICS ; HIGH-RESOLUTION ; HUMAN SERUM ; FC ; GLYCOPEPTIDES ; ACCURATE ; GLYCANS
Abstract	Monoclonal antibodies (mAbs), are one of the most important protein drugs have attracted increasing attention. However, the pharmacokinetics of mAbs has not been fully investigated due to the complexity of protein drugs. Traditonal immuno-based approaches can not recognize the proteoforms of mAbs because of the long development cycles, prohibitive cost, and interactions between different proteins. Therefore, reliable qualitative and quantitative analysis of the proteoforms of mAbs in biological samples is of crucial importance. Herein, a novel method was developed for absolute quantitation of mAbs and their glycoforms in complex biological samples such as serum and tissues. With the combination of HILIC enrichment and parallel reaction monitoring by high resolution mass spectrometry, most of the glycoforms can be accurately quantified at the fmol level through the use of the model mAb of bevacizumab. More importantly, the structural confirmation can be achieved simultaneously without the need for additional experiments. This strategy can be readily applied to the pharmacokinetic study of glycosylation modification and biomarker discovery for clinical applications.
Language	英语
WOS ID	WOS:000394064700094
Publisher	ELSEVIER SCIENCE BV
Citation statistics
Document Type	期刊论文
Identifier	http://cas-ir.dicp.ac.cn/handle/321008/169567
Collection	中国科学院大连化学物理研究所
Corresponding Author	Hu, Lianghai; Ye, Mingliang
Affiliation	1.Jilin Univ, Sch Life Sci, Changchun 130012, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation GB/T 7714	Cong, Yuting,Hu, Lianghai,Zhang, Zhang,et al. Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study[J]. TALANTA,2017,165:664-670.
APA	Cong, Yuting.,Hu, Lianghai.,Zhang, Zhang.,Gao, Yin.,Dong, Mingming.,...&Zou, Hanfa.(2017).Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study.TALANTA,165,664-670.
MLA	Cong, Yuting,et al."Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study".TALANTA 165(2017):664-670.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院大连化学物理研究所机构知识库