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Online Three Dimensional Liquid Chromatography/Mass Spectrometry Method for the Separation of Complex Samples
Wang, Shuangyuan1,2; Shi, Xianzhe1; Xu, Guowang1
Source PublicationANALYTICAL CHEMISTRY
2017-02-07
ISSN0003-2700
DOI10.1021/acs.analchem.6b04401
Volume89Issue:3Pages:1433-1438
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; GAS-CHROMATOGRAPHY ; LIPID ANALYSIS ; WASTE-WATER ; QUANTIFICATION ; IDENTIFICATION ; 2ND-DIMENSION ; COMBINATION ; SAPONINS ; GRADIENT
AbstractIn this work, a novel online three dimensional liquid chromatography (3D-LC) system was first developed by effectively coupling of preseparation and comprehensive 2D-LC using a stop-flow interface, aiming at improving the separation of complex samples. The sample was separated into two or several fractions through the first dimensional separation, and then each fraction was transferred in an orderly way into the following comprehensive 2D-LC part for further analysis. More optimal conditions could be operated in the second and third dimensions according to the properties of each fraction. Thus, the resolution of the 3D-LC system was substantially improved. Analysis of soybean extract was taken as a proof-of-principle to demonstrate the powerful separation of the established 3D-LC system. The amide column was selected as the first dimension column. Weakly polar metabolites (such as lipids, aglycones, etc.) and polar metabolites (such as glycosides, etc.) were separated into different fractions. Fluorophenyl and C18 columns were used in the second and third dimensions of the 3D-LC system for further separation, respectively. There were 83 flavonoids characterized in the soybean extract, including many difficult to separate isomers and low-abundance flavonoids; in total, they were nearly 30% more than those identified in the comparative comprehensive 2D-LC approach. In conclusion, this 3D-LC system is flexible in construction and applicable to complex sample analysis.
Language英语
WOS IDWOS:000393738300009
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169623
Collection中国科学院大连化学物理研究所
Corresponding AuthorShi, Xianzhe; Xu, Guowang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shuangyuan,Shi, Xianzhe,Xu, Guowang. Online Three Dimensional Liquid Chromatography/Mass Spectrometry Method for the Separation of Complex Samples[J]. ANALYTICAL CHEMISTRY,2017,89(3):1433-1438.
APA Wang, Shuangyuan,Shi, Xianzhe,&Xu, Guowang.(2017).Online Three Dimensional Liquid Chromatography/Mass Spectrometry Method for the Separation of Complex Samples.ANALYTICAL CHEMISTRY,89(3),1433-1438.
MLA Wang, Shuangyuan,et al."Online Three Dimensional Liquid Chromatography/Mass Spectrometry Method for the Separation of Complex Samples".ANALYTICAL CHEMISTRY 89.3(2017):1433-1438.
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