DICP OpenIR
Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium
Liu, Peng1; Weng, Yejing2; Sui, Zhigang2; Wu, Yunhao1; Meng, Xiangli1; Wu, Mengwei1; Jin, Haoyi1; Tan, Xiaodong1; Zhang, Lihua2; Zhang, Yukui2
Source PublicationSCIENTIFIC REPORTS
2016-11-21
ISSN2045-2322
DOI10.1038/srep37606
Volume6
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordINTEGRATIVE ANALYSIS ; METASTASIS ; INVOLVEMENT ; BIOMARKERS ; ENRICHMENT ; NETWORKS ; PROTEINS ; PROTEOME ; INVASION ; ANTIGEN
AbstractPancreatic cancer is a highly metastatic and chemo-resistant disease. Secreted proteins involved in cell-cell interactions play an important role in changing the tumor microenvironment. Previous studies generally focus on the secretome of cancer cell line from serum-free media, due to the serious interference of fetal bovine serum (FBS). However, serum-starvation may alter expression patterns of secreted proteins. Hence, efforts to decrease the interference of serum in proteomic analysis of serum-containing media have been hampered to quantitatively measure the tumor secretion levels. Recently, the metabolic labeling, protein equalization, protein fractionation and filter-aided sample preparation (FASP) strategy (MLEFF) has been successfully used to avoid the disturbance of serum on secretome analysis. Here, this efficient method was applied for comparative secretome analysis of two hamster pancreatic cancer cells with differentially metastatic potentials, enabling the observation of 161 differentially expressed proteins, including 106 proteins that had been previously reported and detected in plasma. By integrated analysis of our data and publicly available bioinformatics resources, we found that a combination panel consisting of CDH3, PLAU, and LFNG might improve the prognosis of overall pancreatic cancer survival. These secreted proteins may serve as a potential therapeutic targets for pancreatic cancer metastasis.
Language英语
WOS IDWOS:000388082500001
PublisherNATURE PUBLISHING GROUP
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169725
Collection中国科学院大连化学物理研究所
Corresponding AuthorTan, Xiaodong; Zhang, Lihua
Affiliation1.China Med Univ, Shengjing Hosp, Dept Gen Surg 1, Shenyang 110004, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Liu, Peng,Weng, Yejing,Sui, Zhigang,et al. Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium[J]. SCIENTIFIC REPORTS,2016,6.
APA Liu, Peng.,Weng, Yejing.,Sui, Zhigang.,Wu, Yunhao.,Meng, Xiangli.,...&Zhang, Yukui.(2016).Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium.SCIENTIFIC REPORTS,6.
MLA Liu, Peng,et al."Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium".SCIENTIFIC REPORTS 6(2016).
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