DICP OpenIR
An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo
Zheng, Huizhen1,4; Li, Shen3; Gao, Meng1,4; Ren, Ying1,4; Zheng, Guoshuang1,4; Xie, Hongguo1; Yu, Weiting1; Wang, Xiuli2; Ma, Xiaojun1
Source PublicationRSC ADVANCES
2016
ISSN2046-2069
DOI10.1039/c6ra19294g
Volume6Issue:96Pages:93288-93297
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordCELL-ADHESION ; SUBSTRATE STIFFNESS ; PANCREATIC-ISLETS ; SURFACE ; BIOCOMPATIBILITY ; CAPSULES ; IMMUNOISOLATION ; PROLIFERATION ; ATTACHMENT ; STABILITY
AbstractIn vivo microcapsule implantation suffers from varying degrees of inflammatory responses. Considering the complications of the implantation process and the individual variation in laboratory animals, a feasible model, constructed in vitro by culturing fibroblasts on the surfaces of different types of microcapsules, is established in the present study. A high consistency is shown between the cell adhesion on beads or microcapsules based on the developed model in vitro and their fibrosis formation after implantation in vivo, which can be used to evaluate the biocompatibility of the microcapsules. Moreover, the relationship between the surface properties of the microcapsules and the cell adhesion behavior was explored using this developed model. It was found that cell adhesion was aggravated with chitosan reaction, but was reduced with alginate neutralization. This was closely related to the variation trend of surface charges, but not surface roughness and the stiffness of alginate-based microcapsules during the process of chitosan coating and alginate neutralization. This model could therefore provide a rapid evaluation and guidance to tailor the optimal surface properties for long-term transplantation.
Language英语
WOS IDWOS:000385631900008
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169795
Collection中国科学院大连化学物理研究所
Corresponding AuthorZheng, Huizhen
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Dalian Med Univ, Coll Basic Med Sci, Dept Histol & Embryol, Dalian 116044, Peoples R China
3.Dalian Med Univ, Dalian Municipal Cent Hosp, Dept Endocrinol & Metab, Dalian 116033, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Huizhen,Li, Shen,Gao, Meng,et al. An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo[J]. RSC ADVANCES,2016,6(96):93288-93297.
APA Zheng, Huizhen.,Li, Shen.,Gao, Meng.,Ren, Ying.,Zheng, Guoshuang.,...&Ma, Xiaojun.(2016).An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo.RSC ADVANCES,6(96),93288-93297.
MLA Zheng, Huizhen,et al."An improved model for exploring the effect of physicochemical properties of alginate-based microcapsules on their fibrosis formation in vivo".RSC ADVANCES 6.96(2016):93288-93297.
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