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Synthesis and evaluation of sulfobetaine zwitterionic polymer bonded stationary phase
Yu, Dongping1,2; Guo, Zhimou1,4; Shen, Aijin1; Yan, Jingyu1; Dong, Xuefang1,2; Jin, Gaowa1; Long, Zhen3; Liang, Lina3; Liang, Xinmiao1,4
KeywordHydrophilic Interaction Chromatography Sulfobetaine Zwitterionic Stationary Phase Reversible Addition-fragmentation Transfer Polymerization Saccharide Separation
Source PublicationTALANTA
2016-12-01
ISSN0039-9140
DOI10.1016/j.talanta.2016.09.003
Volume161Pages:860-866
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordINTERACTION LIQUID-CHROMATOGRAPHY ; HYDROPHILIC INTERACTION CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; MONOCLONAL-ANTIBODIES ; BREAST-CANCER ; SEPARATION ; SILICA ; MODE ; FOOD
AbstractZwitterionic polymer stationary phases have attracted increasing attention in hydrophilic interaction chromatography (HILIC). In this work, a zwitterionic sulfobetaine functionalized polyacrylamide stationary phase (named TENS) based on porous silica particles was prepared via controlled surface initiated reversible addition fragmentation transfer (RAFT) polymerization. Instead of traditional methacrylate type sulfobetaine monomer, acrylamide type sulfobetaine monomer, which has higher chemical stability and hydrophicility, was employed in this work. The characterization of elemental analysis and solid-state C-13 cross polarization/magic-anglespinning nuclear magnetic resonance indicated the successful preparation of TENS stationary phase. Meanwhile, scanning electron microscope (SEM), nitrogen adsorption experiment and study of size exclusion performance were conducted, revealing that the surface initiated polymerization was well controlled. For better understanding of TENS material under HILIC mode, chromatographic evaluation of TENS material was performed, among which, TENS material exhibited good hydrophilicity and chemical stability. To further study the applicability of TENS material, saccharides which were considered as challenging targets in HILIC, were chosen as tested analytes. Various saccharide samples, including fructooligosaccharide, trisaccharide isomers and ginsenosides, were well separated on TENS material. Moreover, TENS material displayed good selectivity for the enrichment of glycopeptides. These results demonstrated the capability of TENS as a promising material in glycomics and glycoproteomics.
Language英语
WOS IDWOS:000386989500114
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/169843
Collection中国科学院大连化学物理研究所
Corresponding AuthorGuo, Zhimou; Liang, Xinmiao
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Thermofisher Sci Corp, Beijing 100080, Peoples R China
4.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China
Recommended Citation
GB/T 7714
Yu, Dongping,Guo, Zhimou,Shen, Aijin,et al. Synthesis and evaluation of sulfobetaine zwitterionic polymer bonded stationary phase[J]. TALANTA,2016,161:860-866.
APA Yu, Dongping.,Guo, Zhimou.,Shen, Aijin.,Yan, Jingyu.,Dong, Xuefang.,...&Liang, Xinmiao.(2016).Synthesis and evaluation of sulfobetaine zwitterionic polymer bonded stationary phase.TALANTA,161,860-866.
MLA Yu, Dongping,et al."Synthesis and evaluation of sulfobetaine zwitterionic polymer bonded stationary phase".TALANTA 161(2016):860-866.
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