DICP OpenIR
Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide
Liu, Jianxi1,2; Yang, Kaiguang1; Shao, Wenya1; Li, Senwu1; Wu, Qi1; Zhang, Shen1; Qu, Yanyan1; Zhang, Lihua1; Zhang, Yukui1
KeywordCore-shell Structures Separation Click Chemistry Nanoparticles Glycopeptides
Source PublicationACS APPLIED MATERIALS & INTERFACES
2016-08-31
ISSN1944-8244
DOI10.1021/acsami.6b06343
Volume8Issue:34Pages:22018-22024
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordFUNCTIONALIZED MAGNETIC NANOPARTICLES ; SELECTIVE ENRICHMENT ; MASS-SPECTROMETRY ; STATIONARY-PHASE ; CHROMATOGRAPHY ; GLYCOSYLATION ; GLYCAN ; DEGLYCOSYLATION ; GLYCOPROTEINS ; ADSORBENTS
AbstractBecause, of the low abundance of glycopeptide in natural biological samples, methods for efficient and selective enrichment of glycopeptides play a significant role in mass spectrometry (MS)-based glycoproteomics. In this study, a novel kind of zwitterionic hydrophilic interaction chromatography polymer particles, namely, poly(N,N-methylenebisacrylamide-co-methacrylic acid)@l-Cys (poly(MBAAm-co-MAA)@L-Cys), for the enrichment of glycopeptides was synthesized by a facile and efficient approach that combined distillation precipitation polymerization (DPP) and "thiol-ene" click reaction. In the DPP approach, residual vinyl groups explored outside the core with high density, then the functional ligand cysteine was immobilized onto the surface of core particles by highly efficient thiol-ene click reaction. Taking advantage of the unique structure of poly(MBAAm-co-MAA)@L-Cys, the resulting particles possess remarkable enrichment selectivity for glycopeptides from the tryptic digested human immunoglobulin G. The polymer particles were successfully employed for the analysis of human plasma, and 208 unique glycopeptides corresponding to 121 glycoproteins were reliably identified in triple independent nano-LC-MS/MS runs. The selectivity toward glycopeptides of these particles poly(MBAAm-co-MAA)@L-Cys is similar to 2 times than that of the commercial beads. These results demonstrated that these particles had great potential for large-scale glycoproteomics research. Moreover, the strategy with the combination of DPP and thiol-ene click chemistry might be a facile method to produce functional polymer particles for bioenrichment application.
Language英语
WOS IDWOS:000382514100022
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170093
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China
2.Fujian Normal Univ, Coll Environm Sci & Engn, Fuzhou 350007, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jianxi,Yang, Kaiguang,Shao, Wenya,et al. Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(34):22018-22024.
APA Liu, Jianxi.,Yang, Kaiguang.,Shao, Wenya.,Li, Senwu.,Wu, Qi.,...&Zhang, Yukui.(2016).Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide.ACS APPLIED MATERIALS & INTERFACES,8(34),22018-22024.
MLA Liu, Jianxi,et al."Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide".ACS APPLIED MATERIALS & INTERFACES 8.34(2016):22018-22024.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Jianxi]'s Articles
[Yang, Kaiguang]'s Articles
[Shao, Wenya]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Jianxi]'s Articles
[Yang, Kaiguang]'s Articles
[Shao, Wenya]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Jianxi]'s Articles
[Yang, Kaiguang]'s Articles
[Shao, Wenya]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.