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Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination
Qing, Guangyan1; Li, Xiuling2; Xiong, Peng1; Chen, Cheng2; Zhan, Mimi1; Liang, Xinmiao2; Sun, Taolei1,3
KeywordPolymer Interface Glycoproteomics Carbohydrate Enrichment
Source PublicationACS APPLIED MATERIALS & INTERFACES
2016-08-31
ISSN1944-8244
DOI10.1021/acsami.6b07863
Volume8Issue:34Pages:22084-22092
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; TITANIUM-DIOXIDE CHROMATOGRAPHY ; PROTEIN GLYCOSYLATION ANALYSIS ; MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; SELECTIVE ENRICHMENT ; HYDRAZIDE CHEMISTRY ; CANCER ; IDENTIFICATION ; RECOGNITION
AbstractGlycoproteomics identifies and catalogs protein glycosylation and explores its impact on protein conformations and biofunctions. However, these studies are restricted by the bottleneck to enrich low-abundance glycopeptides from complex biosamples and the difficulties in analyzing glycan structures by mass spectrometry. Here, we report dipeptide as a simple but promising carbohydrate binding platform to tackle these problems. We build a hydropathy-index-based strategy for sequence optimization and screen out three optimal dipeptide sequences from 54 types of dipeptides. The optimized dipeptide-based homopolymers display excellent performance (e.g., selectivity up to similar to 70% for real biosamples and strong anti-interference capacity capable of resisting 1000-fold bovine serum albumin interference) glycopeptide enrichment. Meanwhile, our polymers exhibit high-efficiency chromatographic separation toward oligosaccharides with different compositions, polymerization degrees and even their linkage isomers. This brings another attractive feature that our materials can discriminate subtly variable glycan structures of glycopeptides, especially, isomeric glycosidic linkages. These features provide a solid foundation to analyze the complex glycan structures and glycosites simultaneously, which will benefit future development of glycoproteomics and glycobiology.
Language英语
WOS IDWOS:000382514100029
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170127
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang, Xinmiao; Sun, Taolei
Affiliation1.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, 122 Luoshi Rd, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, 122 Luoshi Rd, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Qing, Guangyan,Li, Xiuling,Xiong, Peng,et al. Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(34):22084-22092.
APA Qing, Guangyan.,Li, Xiuling.,Xiong, Peng.,Chen, Cheng.,Zhan, Mimi.,...&Sun, Taolei.(2016).Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination.ACS APPLIED MATERIALS & INTERFACES,8(34),22084-22092.
MLA Qing, Guangyan,et al."Dipeptide-Based Carbohydrate Receptors and Polymers for Glycopeptide Enrichment and Glycan Discrimination".ACS APPLIED MATERIALS & INTERFACES 8.34(2016):22084-22092.
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