DICP OpenIR
The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer
Zhou, Yang1,2; Song, Ruixiang3; Zhang, Zhensheng3; Lu, Xin1; Zeng, Zhongda1; Hu, Chunxiu1; Liu, Xinyu1,2; Li, Yanli1; Hou, Jianguo3; Sun, Yinghao3; Xu, Chuanliang3; Xu, Guowang1
KeywordMetabolomics Bladder Cancer Metabolic Profiling Gas Chromatography-mass Spectrometry Pseudotargeted Plasma
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2016-09-01
ISSN1618-2642
DOI10.1007/s00216-016-9797-0
Volume408Issue:24Pages:6741-6749
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordGAS CHROMATOGRAPHY/MASS SPECTROMETRY ; PENTOSE-PHOSPHATE PATHWAY ; MASS SPECTROMETRY ; BIOMARKER DISCOVERY ; CROSS-VALIDATION ; TOBACCO-LEAVES ; EXPRESSION ; URINARY ; CLASSIFICATION ; PROGRESSION
AbstractBladder cancer (BC) is a fatal malignancy with considerable mortality. BC urinary metabolomics has been extensively investigated for biomarker discovery, but few BC blood metabolomic studies have been performed. Hence, a plasma pseudotargeted metabolomic method based on gas chromatography-mass spectrometry with selected ion monitoring (GC-MS-SIM) was developed to study metabolic alterations in BC. The analytical performance of the developed method was compared with that of a nontargeted method. The relative standard deviation (RSD) values of 89 and 70.7 % of the peaks obtained using the pseudotargeted and nontargeted methods, respectively, were less than 20 %. The Pearson correlations of 90.7 and 78.3 % of the peaks obtained using the pseudotargeted and nontargeted methods, respectively, exceeded 0.90 in the linearity evaluation. Compared with the nontargeted method, the signal-to-noise ratios (S/N) of 97.9 and 69.3 % of the peaks increased two- and fivefold, respectively. The developed method was fully validated, with good precision, recovery, and stability of the trimethylsilyl (TMS) derivatives. The method was applied to investigate BC. Significant increases in the contents of metabolites involved in, for example, the pentose phosphate pathway (PPP) and nucleotide and fatty acid synthesis were found in the high-grade (HG) BC group compared to the healthy control (HC) group. These differences imply that the activated PPP may regulate BC cell proliferation by promoting lipid and nucleotide biosynthesis and the detoxification of reactive oxygen species (ROS). These results illustrate that the plasma pseudotargeted method is a powerful tool for metabolic profiling.
Language英语
WOS IDWOS:000382883300021
PublisherSPRINGER HEIDELBERG
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170257
Collection中国科学院大连化学物理研究所
Corresponding AuthorLu, Xin; Xu, Chuanliang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Secondary Mil Med Univ, Shanghai Changhai Hosp, Dept Urol, Shanghai 200433, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Yang,Song, Ruixiang,Zhang, Zhensheng,et al. The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(24):6741-6749.
APA Zhou, Yang.,Song, Ruixiang.,Zhang, Zhensheng.,Lu, Xin.,Zeng, Zhongda.,...&Xu, Guowang.(2016).The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,408(24),6741-6749.
MLA Zhou, Yang,et al."The development of plasma pseudotargeted GC-MS metabolic profiling and its application in bladder cancer".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408.24(2016):6741-6749.
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