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Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy
Liu, Zheyi1,2; Wang, Fangjun1; Chen, Jin1,2; Zhou, Ye1,2; Zou, Hanfa1
KeywordMulti-phosphopeptide Enrichment Competitive Substitution Pre-coordination Mass Spectrometry Phosphoproteomics
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2016-08-26
ISSN0021-9673
DOI10.1016/j.chroma.2016.07.042
Volume1461Pages:35-41
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPHOSPHORYLATED PEPTIDES ; PROTEIN-PHOSPHORYLATION ; MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; ENRICHMENT ; PHOSPHOPROTEOME ; CHROMATOGRAPHY ; SEPARATION ; NETWORKS ; TIO2
AbstractAlthough many affinity adsorbents have been developed for phosphopeptides enrichment, high specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ion coordination and substitution on affinity adsorbents surfaces and modulated the selectivity of affinity adsorbents to multi-phosphopeptides based on the different capability of mono and multi-phosphopeptides in competitively substituting the pre-coordinated phosphate ions at strong acidic condition. We demonstrated both the species of pre-coordinated phosphate ions and the substituting conditions played crucial roles in modulating the enrichment selectivity to multi-phosphopeptides, and the pre-coordinated affinity materials with relative more surfaces positive charges exhibited better enrichment efficiency due to the cooperative effect of electrostatic interaction and competitive substitution. Finally, an enrichment selectivity of 85% to multi-phosphopeptides was feasibly achieved with 66% improvement in identification numbers for complex protein sample extracted from HepG2 cells. Data are available via ProteomeXchange with identifier PXD004252. (c) 2016 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000381840200005
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170301
Collection中国科学院大连化学物理研究所
Corresponding AuthorWang, Fangjun
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zheyi,Wang, Fangjun,Chen, Jin,et al. Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1461:35-41.
APA Liu, Zheyi,Wang, Fangjun,Chen, Jin,Zhou, Ye,&Zou, Hanfa.(2016).Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy.JOURNAL OF CHROMATOGRAPHY A,1461,35-41.
MLA Liu, Zheyi,et al."Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy".JOURNAL OF CHROMATOGRAPHY A 1461(2016):35-41.
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