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NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition
Qi, Xiaoyue1; Chen, Long1; Zhang, Chaoqun2; Xu, Xinyuan1; Zhang, Yiding1; Bai, Yu1; Liu, Huwei1
KeywordAffinity Probes His-tagged Proteins Purification Phosphopeptides Enrichment Metal Oxide Buffer Modulation
Source PublicationACS Applied Materials & Interfaces
2016-07-27
ISSN1944-8244
DOI10.1021/acsami.6b04280
Volume8Issue:29Pages:18675-18683
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordPHOSPHOPEPTIDE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; ELECTRICAL-PROPERTIES ; SELECTIVE ENRICHMENT ; MAGNETIC SEPARATION ; NANOPARTICLES ; NANOSPHERES ; ACTIVATION ; REGULATOR ; AUTOPHAGY
AbstractA bifunctional affinity probe NiCoMnO4 was designed and prepared with controllable morphology and size using facile methods. It was observed that the probe could be applied in His-tagged proteins purification and phosphopeptides enrichment simply through the buffer modulation. NiCoMnO4 particles showed satisfactory cycling performance for His-tagged proteins purification and broad pH-tolerance of loading buffer for phosphopeptides affinity. Therefore, a high-throughput, cost-effective, and efficient protein/peptide purification method was developed within 10 min based on the novel bifunctional affinity probe.
Language英语
WOS IDWOS:000380735500006
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170327
Collection中国科学院大连化学物理研究所
Corresponding AuthorBai, Yu; Liu, Huwei
Affiliation1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Beijing Nucl Magnet Resonance Ctr, Coll Life Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Qi, Xiaoyue,Chen, Long,Zhang, Chaoqun,et al. NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition[J]. ACS Applied Materials & Interfaces,2016,8(29):18675-18683.
APA Qi, Xiaoyue.,Chen, Long.,Zhang, Chaoqun.,Xu, Xinyuan.,Zhang, Yiding.,...&Liu, Huwei.(2016).NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition.ACS Applied Materials & Interfaces,8(29),18675-18683.
MLA Qi, Xiaoyue,et al."NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition".ACS Applied Materials & Interfaces 8.29(2016):18675-18683.
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