DICP OpenIR
Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry
Zhang, Yun1; Jin, Hongli1; Li, Xiaolong1; Zhao, Jianqiang2; Guo, Xiujie1; Wang, Jixia1; Guo, Zhimou1; Zhang, Xiuli1; Tao, Yanduo2; Liu, Yanfang1; Chen, Deliang3; Liang, Xinmiao1
KeywordBufadienolides Normal-phase Liquid Chromatography Two-dimensional Liquid Chromatography Ms/ms Toad Skin
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2016-07-15
ISSN1570-0232
DOI10.1016/j.jchromb.2015.11.015
Volume1026Pages:67-74
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordBUFO-GARGARIZANS CANTOR ; EFFICIENT PURIFICATION ; CARDIAC-GLYCOSIDES ; CHAN-SU ; ACTIVE BUFADIENOLIDES ; CELL-GROWTH ; HPLC-DAD ; STEROIDS ; CANCER ; IDENTIFICATION
AbstractBufadienolides possess various bioactivities especially antitumor. Due to the high structural diversity, the separation of bufadienolides often suffers from coelution problem on conventional RP columns. In this work, an off-line two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography (2D-NPLC x RPLC) method was developed to separate and characterize bufadienolides in toad skin. Several RP and NP columns were evaluated with five reference bufadienlides. The XUnion C18 and XAmide columns exhibited superior chromatographic performances for bufadienlide separation, and were selected in RPLC and NPLC, respectively. RPLC was used in the second-dimension for the good compatibility with MS, while NPLC was adopted in the first-dimension. The orthogonality of the 2D-NPLC x RPLC system was investigated by the geometric approach using fifteen bufadienolide mixtures. The result was 49.6%, demonstrating reasonable orthogonality of this 2D-LC system. By combining the 2D-LC system with MS, 64 bufadienlides including 33 minor ones and 11 pairs of isomers in toad skin were identified. This off-line 2D-NPLC x RPLC allowed to solve the coelution problem of bufadienlides in one-dimension RPLC, and thus facilitated the identification significantly. (C) 2015 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000379636800008
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170425
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Yanfang; Chen, Deliang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China
3.Wuhan Univ, Zhongnan Hosp, Dept Internal Med, Div Cardiol, Wuhan 430071, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yun,Jin, Hongli,Li, Xiaolong,et al. Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1026:67-74.
APA Zhang, Yun.,Jin, Hongli.,Li, Xiaolong.,Zhao, Jianqiang.,Guo, Xiujie.,...&Liang, Xinmiao.(2016).Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1026,67-74.
MLA Zhang, Yun,et al."Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1026(2016):67-74.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Yun]'s Articles
[Jin, Hongli]'s Articles
[Li, Xiaolong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Yun]'s Articles
[Jin, Hongli]'s Articles
[Li, Xiaolong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Yun]'s Articles
[Jin, Hongli]'s Articles
[Li, Xiaolong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.