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Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides
Liu, Yujie1,2; Fu, Dongmei2; Yu, Long2,3; Xiao, Yuansheng2; Peng, Xiaojun1; Liang, Xinmiao2,3
KeywordGlycoprotein/glycopeptide Lectin Affinity Chromatography Mass Spectrometry Oxidized Dextran
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2016-07-15
ISSN0021-9673
DOI10.1016/j.chroma.2016.05.093
Volume1455Pages:147-155
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMASS-SPECTROMETRIC ANALYSIS ; YEAST EXTERNAL INVERTASE ; GLYCOSYLATION SITES ; HUMAN SERUM ; CHROMATOGRAPHY ; GLYCOPROTEINS ; STRATEGIES ; OVALBUMIN ; SUPPORTS ; IDENTIFICATION
AbstractLectin affinity chromatography (LAC) is an important enrichment technique in glycoproteomics analysis. In order to improve the effectiveness of enrichment, it is necessary to develop LAC materials with high specificity and efficiency. Herein, using oxidized dextran as the spacer, a silica-based concanavalin A material (SiO2-ODex Con A) was synthesized to enrich glycoproteins/glycopeptides. For comparison, the SiO2-Ald Con A material was synthesized using conventional (3-glycidoxypropyl) triethoxysilane (GPMS) as the initial spacer arm. The analytical merits of both Con A materials, such as non-specific adsorption, binding capacity and trapping efficiency, have been evaluated using ovalbumin. Under high performance liquid affinity chromatography (HPLAC) mode, the SiO2-ODex Con A material was highly effective in the enrichment of glycoproteins/glycopeptides attached to high-mannose-type and bi-antennary complex type glycans. The promising potential of the SiO2-ODex Con A material was demonstrated by selective fractionation of glycoproteins from complex biological samples for glycosylation analysis. (C) 2016 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000378954900017
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170463
Collection中国科学院大连化学物理研究所
Corresponding AuthorYu, Long; Liang, Xinmiao
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yujie,Fu, Dongmei,Yu, Long,et al. Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1455:147-155.
APA Liu, Yujie,Fu, Dongmei,Yu, Long,Xiao, Yuansheng,Peng, Xiaojun,&Liang, Xinmiao.(2016).Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides.JOURNAL OF CHROMATOGRAPHY A,1455,147-155.
MLA Liu, Yujie,et al."Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides".JOURNAL OF CHROMATOGRAPHY A 1455(2016):147-155.
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