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Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase
Zhu, Benwei1,2; Chen, Meijuan1; Yin, Heng3; Du, Yuguang4; Ning, Limin1
KeywordAlginate Lyase Cellulophaga Enzymatic Hydrolysis Oligosaccharides
Source PublicationMARINE DRUGS
2016-06-01
ISSN1660-3397
DOI10.3390/md14060108
Volume14Issue:6
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectChemistry, Medicinal
WOS Research AreaPharmacology & Pharmacy
WOS KeywordOLIGOALGINATE LYASE ; CYTOKINE PRODUCTION ; DEGRADING ENZYMES ; PURIFICATION ; MACROPHAGES ; EXPRESSION ; INDUCTION ; CLONING ; CELLS
AbstractEnzymatic hydrolysis of sodium alginate to produce alginate oligosaccharides has drawn increasing attention due to its advantages of containing a wild reaction condition, excellent gel properties and specific products easy for purification. However, the efficient commercial enzyme tools are rarely available. A new alginate lyase with high activity (24,038 U/mg) has been purified from a newly isolated marine strain, Cellulophaga sp. NJ-1. The enzyme was most active at 50 degrees C and pH 8.0 and maintained stability at a broad pH range (6.0-10.0) and temperature below 40 degrees C. It had broad substrate specificity toward sodium alginate, heteropolymeric MG blocks (polyMG), homopolymeric M blocks (polyM) and homopolymeric G blocks (polyG), and possessed higher affinity toward polyG (15.63 mM) as well as polyMG (23.90 mM) than polyM (53.61 mM) and sodium alginate (27.21 mM). The TLC and MS spectroscopy analysis of degradation products suggested that it completely hydrolyzed sodium alginate into oligosaccharides of low degrees of polymerization (DPs). The excellent properties would make it a promising tool for full use of sodium alginate to produce oligosaccharides.
Language英语
WOS IDWOS:000378770300005
PublisherMDPI AG
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170489
Collection中国科学院大连化学物理研究所
Corresponding AuthorNing, Limin
Affiliation1.Nanjing Univ Chinese Med, Preclin Med Coll, Nanjing 210023, Jiangsu, Peoples R China
2.Nanjing Univ Technol, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Benwei,Chen, Meijuan,Yin, Heng,et al. Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase[J]. MARINE DRUGS,2016,14(6).
APA Zhu, Benwei,Chen, Meijuan,Yin, Heng,Du, Yuguang,&Ning, Limin.(2016).Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase.MARINE DRUGS,14(6).
MLA Zhu, Benwei,et al."Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase".MARINE DRUGS 14.6(2016).
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