DICP OpenIR
A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals
Jin, Qiang1,2; Feng, Lei3; Wang, Dan-Dan1; Wu, Jing-Jing1; Hou, Jie3,4; Dai, Zi-Ru1; Sun, Shi-Guo3; Wang, Jia-Yue1; Ge, Guang-Bo1,3; Cui, Jing-Nan3; Yang, Ling1
KeywordNir Fluorescent Probe Carboxylesterase 2 Enzymatic Activity Specific Detection Bioimaging
Source PublicationBIOSENSORS & BIOELECTRONICS
2016-09-15
ISSN0956-5663
DOI10.1016/j.bios.2016.04.075
Volume83Pages:193-199
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordIN-VIVO ; FAR-RED ; SMALL-INTESTINE ; HUMAN LIVER ; ACTIVATION ; EXPRESSION ; PRODRUG ; FLUOROPHORE ; METABOLISM ; IRINOTECAN
AbstractA near-infrared fluorescent probe (DDAB) for highly selective and sensitive detection of carboxylesterase 2 (CE2) has been designed, synthesized, and systematically studied both in vitro and in vivo. Upon addition of CE2, the ester bond of DDAB could be rapidly cleaved and then release a near-infrared (NIR) fluorophore DDAO, which brings a remarkable yellow-to-blue color change and strong NIR fluorescence emission in physiological solutions. The newly developed probe exhibits excellent properties including good specificity, ultrahigh sensitivity and high imaging resolution. Moreover, DDAB has been applied to measure the real activities of CE2 in complex biological samples, as well as to screen CE2 inhibitors by using tissue preparations as the enzymes sources. The probe has also been successfully used to detect endogenous CE2 in living cells and in vivo for the first time, and the results demonstrate that such detection is highly reliable. All these prominent features of DDAB make it holds great promise for further investigation on CE2-associated biological process and for exploring the physiological functions of CE2 in living systems. (C) 2016 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000376802300027
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170599
Collection中国科学院大连化学物理研究所
Corresponding AuthorGe, Guang-Bo; Cui, Jing-Nan; Yang, Ling
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
4.Dalian Med Univ, Dalian 116044, Peoples R China
Recommended Citation
GB/T 7714
Jin, Qiang,Feng, Lei,Wang, Dan-Dan,et al. A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals[J]. BIOSENSORS & BIOELECTRONICS,2016,83:193-199.
APA Jin, Qiang.,Feng, Lei.,Wang, Dan-Dan.,Wu, Jing-Jing.,Hou, Jie.,...&Yang, Ling.(2016).A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals.BIOSENSORS & BIOELECTRONICS,83,193-199.
MLA Jin, Qiang,et al."A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals".BIOSENSORS & BIOELECTRONICS 83(2016):193-199.
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