DICP OpenIR
Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening
Wang, Jian-Zheng1,2,3; Zhu, Yu-Xia1,2,3; Ma, Hui-Chao2,3; Chen, Si-Nan2,3; Chao, Ji-Ye2,3; Ruan, Wen-Ding2,3; Wang, Duo2,3; Du, Feng-guang2,3; Meng, Yue-Zhong4
KeywordThree-dimensional Cell Culture Multi-cellular Tumor Spheroid (Mots) Spray-spinning Chitosan/collagen/alginate Scaffold (Cca)
Source PublicationMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
2016-05-01
ISSN0928-4931
DOI10.1016/j.msec.2016.01.045
Volume62Pages:215-225
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectMaterials Science, Biomaterials
WOS Research AreaMaterials Science
WOS KeywordMESENCHYMAL STEM-CELLS ; CANCER-CELL ; MULTICELLULAR SPHEROIDS ; CHITOSAN-ALGINATE ; MAMMALIAN TARGET ; EPITHELIAL-CELLS ; BREAST-CANCER ; MASS-TRANSFER ; CULTURE ; BONE
AbstractIn this work, a 3D MCTS-CCA system was constructed by culturing multi-cellular tumor spheroid (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. The CCA scaffolds were fabricated by spray-spinning. The interactions between the components of the spray-spun fibers were evidenced by methods of Coomassie Blue stain, X-ray diffraction (XRD) and Fourier transform-infrared spectroscopy (FTIR). Co-culture indicated that MCF-7 cells showed a spatial growth pattern of multi-cellular tumor spheroid (MCTS) in the CCA fibrous scaffold with increased proliferation rate and drug-resistance to MMC, ADM and 5-Aza comparing with the 2D culture cells. Significant increases of total viable cells were found in 3D MCTS groups after drug administration by method of apoptotic analysis. Glucose-lactate analysis indicated that the metabolism of MCTS in CCA scaffold was closer to the tumor issue in vivo than the monolayer cells. In addition, MCTS showed the characteristic of epithelial mesenchymal transition (EMT) which is subverted by carcinoma cells to facilitate metastatic spread. These results demonstrated that MCTS in CCA scaffold possessed a more conservative phenotype of tumor than monolayer cells, and anticancer drug screening in 3D MCTS-CCA system might be superior to the 2D culture system. (C) 2016 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000372759100027
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170643
Collection中国科学院大连化学物理研究所
Corresponding AuthorWang, Jian-Zheng
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Tianguan Grp Co Ltd, Affiliated Gen Hosp, Nanyang 473000, Peoples R China
3.Testing Ctr Henan Tianguan Grp Co Ltd, 1 Tianguan Rd, Nanyang 473000, Henan Province, Peoples R China
4.Sun Yat Sen Univ, State Key Lab Optoelect Mat & Technol, Guangzhou 510275, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jian-Zheng,Zhu, Yu-Xia,Ma, Hui-Chao,et al. Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2016,62:215-225.
APA Wang, Jian-Zheng.,Zhu, Yu-Xia.,Ma, Hui-Chao.,Chen, Si-Nan.,Chao, Ji-Ye.,...&Meng, Yue-Zhong.(2016).Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,62,215-225.
MLA Wang, Jian-Zheng,et al."Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 62(2016):215-225.
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