DICP OpenIR
Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin
Chen, Yuanbo1,2; Deng, Nan1,2; Wu, Ci1,2; Liang, Yu1; Jiang, Bo1; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
KeywordAptamer Hydrophilic Monolith Human Alpha-thrombin Trace Protein Detection
Source PublicationTALANTA
2016-07-01
ISSN0039-9140
DOI10.1016/j.talanta.2016.02.054
Volume154Pages:555-559
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordIN-VITRO SELECTION ; AFFINITY-CHROMATOGRAPHY ; STATIONARY-PHASE ; CAPILLARY CHROMATOGRAPHY ; MAGNETIC BEADS ; ADENOSINE ; PROTEINS ; CAPTURE ; RECOGNITION ; EXTRACTION
AbstractLow abundant proteins of body fluids participate nearly all physiological processes and indicate various kinds of diseases. The development of specific enrichment techniques is the key to identify and quantify the low abundant proteins. Herein, a novel kind of aptamer functionalized hydrophilic polymer monolith was developed for the specific enrichment and detection of human a -thrombin from the human plasma. Human a -thrombin aptamer, with thiol group modified at the 5' terminal, was immobilized on the gold nanoparticles (AuNPs) modified poly(glycidyl methacrylate-co-poly(ethylene glycol) diacrylate) monolithic column, with the binding capacity of 277.1 ilmol/L. Due to the hydrophilic poly(ethylene glycol) diacrylate) as the cross-linking monomer, the detection recovery of the aptamer-functionalized hydrophilic polymer monolithic column could reach to 92.6 +/- 5.2% (n=3) and the dynamic range could reach 0.5-300 ng/mu L (S/N > 10) with on-line UV detection. Meanwhile, the column could run over 100 times, because the poly(glycidyl methacrylate-co-poly(ethylene glycol) diacrylate) stability structure and the AuNPs improved the stability of the matrix material. Furthermore, this column could even capture the target alpha-thrombin, which was spiked in 1000 folds of original human plasma. All these results demonstrated the great potential of the prepared aptamer functionalized hydrophilic polymer monolith for the recognition of the trace proteins in the biological samples. (C) 2016 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000376696400074
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/170975
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Chen, Yuanbo,Deng, Nan,Wu, Ci,et al. Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin[J]. TALANTA,2016,154:555-559.
APA Chen, Yuanbo.,Deng, Nan.,Wu, Ci.,Liang, Yu.,Jiang, Bo.,...&Zhang, Yukui.(2016).Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin.TALANTA,154,555-559.
MLA Chen, Yuanbo,et al."Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human alpha-thrombin".TALANTA 154(2016):555-559.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Yuanbo]'s Articles
[Deng, Nan]'s Articles
[Wu, Ci]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Yuanbo]'s Articles
[Deng, Nan]'s Articles
[Wu, Ci]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Yuanbo]'s Articles
[Deng, Nan]'s Articles
[Wu, Ci]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.