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Improving permeability and chromatographic performance of poly(pentaerythritol diacrylate monostearate) monolithic column via photo-induced thiol-acrylate polymerization
Wang, Hongwei1,2,3; Ou, Junjie2; Bai, Jingyao2; Liu, Zhongshan2,3; Yao, Yating2,3; Chen, Lianfang2,3; Peng, Xiaojun1; Zou, Hanfa2
KeywordPolymeric Monoliths Pentaerythritol Diacrylate Monostearate Trimethyloipropane Tris(3-mercaptopropionate) Thiol-acrylate Polymerization Photoinitiated 2 2-dimethoxy-2-phenylacetophenone
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2016-03-04
ISSN0021-9673
DOI10.1016/j.chroma.2016.01.063
Volume1436Pages:100-108
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCAPILLARY LIQUID-CHROMATOGRAPHY ; RETENTION-INDEPENDENT PERFORMANCE ; LARGE SURFACE-AREA ; STATIONARY PHASES ; SMALL MOLECULES ; REVERSED-PHASE ; ELECTROCHROMATOGRAPHIC CHARACTERIZATION ; METHACRYLATE MONOLITHS ; HYBRID MONOLITHS ; CHARGED SOLUTES
AbstractA simple approach was developed for rapid preparation of polymeric monolithic columns in UV transparent fused-silica capillaries via photoinitiated thiol-acrylate polymerization of pentaerythritol diacrylate monostearate (PEDAS) and trimethylolpropane tris(3-mercaptopropionate) (TPTM) within 10 min, in which the acrylate homopolymerized and copolymerized with the thiol simultaneously. The morphology, permeability and chromatographic performance of the resulting poly(PEDAS-co-TPTM) monoliths were studied. It could be observed from SEM that the morphology of poly(PEDAS-co-TPTM) monolith was rather different from that of poly(PEDAS) monolith, which was fabricated via photo-induced free radical polymerization using PEDAS as the sole monomer. Compared with poly(PEDAS) monolith, poly(PEDAS-co-TPTM) monolith possessed better permeability when they were fabricated under the same preparation conditions. By adjusting the composition of porogenic solvents, poly(PEDAS-co-TPTM) monolith exhibited lower plate heights (15.7-17.7 mu m) than poly(PEDAS) monolith (19.1-37.9 mu m) in mu LC. In addition, 66 unique peptides were positively identified on poly(PEDAS-co-TPTM) monolith when tryptic digest of four proteins was separated by mu LC-MS/MS, demonstrating its potential in proteome analysis. (C) 2016 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000371654700013
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171055
Collection中国科学院大连化学物理研究所
Corresponding AuthorOu, Junjie; Zou, Hanfa
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hongwei,Ou, Junjie,Bai, Jingyao,et al. Improving permeability and chromatographic performance of poly(pentaerythritol diacrylate monostearate) monolithic column via photo-induced thiol-acrylate polymerization[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1436:100-108.
APA Wang, Hongwei.,Ou, Junjie.,Bai, Jingyao.,Liu, Zhongshan.,Yao, Yating.,...&Zou, Hanfa.(2016).Improving permeability and chromatographic performance of poly(pentaerythritol diacrylate monostearate) monolithic column via photo-induced thiol-acrylate polymerization.JOURNAL OF CHROMATOGRAPHY A,1436,100-108.
MLA Wang, Hongwei,et al."Improving permeability and chromatographic performance of poly(pentaerythritol diacrylate monostearate) monolithic column via photo-induced thiol-acrylate polymerization".JOURNAL OF CHROMATOGRAPHY A 1436(2016):100-108.
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