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A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues
Jin, Qiang1; Feng, Lei2; Wang, Dan-Dan1; Dai, Zi-Ru1; Wang, Ping1; Zou, Li-Wei1; Liu, Zhi-Hong3; Wang, Jia-Yue1; Yu, Yang1; Ge, Guang-Bo1,2; Cui, Jing-Nan2; Yang, Ling1
KeywordTwo-photon Ratiometric Fluorescent Probe Carboxylesterase 2 Specific Sensing Bioimaging
Source PublicationACS APPLIED MATERIALS & INTERFACES
2015-12-30
ISSN1944-8244
DOI10.1021/acsami.5b09573
Volume7Issue:51Pages:28474-28481
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordLIVER CARBOXYLESTERASE ; SMALL-INTESTINE ; TUMOR-TISSUE ; MICROSCOPY ; PRODRUG ; HYDROLYSIS ; ACTIVATION ; CAPECITABINE ; MITOCHONDRIA ; IRINOTECAN
AbstractIn this study, a two-photon ratiometric fluorescent probe NCEN has been designed and developed for highly selective and sensitive sensing of human carboxylesterase 2 (hCE2) based on the catalytic properties and substrate preference of hCE2. Upon addition of hCE2, the probe could be readily hydrolyzed to release 4-amino-1,8-naphthalimide (NAH), which brings remarkable red-shift in fluorescence (90 nm) spectrum. The newly developed probe exhibits good specificity, ultrahigh sensitivity, and has been successfully applied to determine the real activities of hCE2 in complex biological samples such as cell and tissue preparations. NCEN has also been used for two-photon imaging of intracellular hCE2 in living cells as well as in deep-tissues for the first time, and the results showed that the probe exhibited high ratiometric imaging resolution and deep-tissue imaging depth. All these findings suggested that this probe holds great promise for applications in bioimaging of endogenous hCE2 in living cells and in exploring the biological functions of hCE2 in complex biological systems.
Language英语
WOS IDWOS:000369448200058
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171181
Collection中国科学院大连化学物理研究所
Corresponding AuthorGe, Guang-Bo
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jin, Qiang,Feng, Lei,Wang, Dan-Dan,et al. A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(51):28474-28481.
APA Jin, Qiang.,Feng, Lei.,Wang, Dan-Dan.,Dai, Zi-Ru.,Wang, Ping.,...&Yang, Ling.(2015).A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues.ACS APPLIED MATERIALS & INTERFACES,7(51),28474-28481.
MLA Jin, Qiang,et al."A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues".ACS APPLIED MATERIALS & INTERFACES 7.51(2015):28474-28481.
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