DICP OpenIR
Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae
Shen, Pei-Li1,2; Wang, Hai-Tao1,2; Pan, Yan-Fei3; Meng, Ying-Ying4; Wu, Pei-Chun1; Xue, Song1
KeywordMicroalgae Triacylglycerols Neutral Lipids Characteristic Fattyacids Biofuels
Source PublicationFRONTIERS IN PLANT SCIENCE
2016-02-22
ISSN1664-462X
DOI10.3389/fpls.2016.00162
Volume7
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectPlant Sciences
WOS Research AreaPlant Sciences
WOS KeywordCHLAMYDOMONAS-REINHARDTII ; NANNOCHLOROPSIS-OCEANICA ; NITROGEN STARVATION ; ARACHIDONIC-ACID ; ACCUMULATION ; BIODIESEL ; LIPIDS ; DESATURASE
AbstractThe fatty acid profiles of lipids from microalgae are unique. Polyunsaturated fatty acids are generally enriched in polar lipids, whereas saturated and monounsaturated fatty acids constitute the majority of fatty acids in triacylglycerols (TAG). Each species has characteristic fatty acids, and their content is positively or negatively correlated with TAGs. The marine oleaginous diatom Phaeodactylum tricornutum was used as the paradigm to determine the quantitative relationship between TAG and characteristic fatty acid content. Fatty acid profiles and TAG content of Phaeodactylum tricornutum were determined in a time course. C16:0/C16:1 and eicosapentaenoic acid (EPA, C20:5n3) were identified as characteristic fatty acids in TAGs and polar lipids, respectively. The percentage of those characteristic fatty acids in total fatty acids had a significant linear relationship with TAG content, and thus, the correlation coefficient presenting r(2) were 0.96, 0.94, and 0.97, respectively. The fatty acid-based method for TAG quantification could also be applied to other microalgae such as Nannochloropsis oceanica in which the r(2) of C16:0 and EPA were 0.94 and 0.97, respectively, and in Chlorella pyrenoidosa-values for C18:1 and C18:3 with TAG content were 0.91 and 0.99, repectively. This characteristic fatty acid-based method provided a distinct way to quantify TAGs in microalgae, by which TAGs could be measured precisely by immediate transesterification from wet biomass rather than using conventional methods. This procedure simplified the operation and required smaller samples than conventional methods.
Language英语
WOS IDWOS:000370543500001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171247
Collection中国科学院大连化学物理研究所
Corresponding AuthorXue, Song
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Marine Bioengn Grp, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Univ, Coll Environm & Chem Engn, Dept Environm Sci & Engn, Dalian 116012, Peoples R China
4.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Shen, Pei-Li,Wang, Hai-Tao,Pan, Yan-Fei,et al. Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA Shen, Pei-Li,Wang, Hai-Tao,Pan, Yan-Fei,Meng, Ying-Ying,Wu, Pei-Chun,&Xue, Song.(2016).Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae.FRONTIERS IN PLANT SCIENCE,7.
MLA Shen, Pei-Li,et al."Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae".FRONTIERS IN PLANT SCIENCE 7(2016).
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