DICP OpenIR
Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition
Wu Shuang; Zhou Jiannan; Cao Xupeng; Xue Song
KeywordChrysophyta Isochrysis Zhangjiangensis Nitrogen Deficiency Lipid Accumulation Reference Gene Selection
Source PublicationJOURNAL OF OCEAN UNIVERSITY OF CHINA
2016-02-01
ISSN1672-5182
DOI10.1007/s11802-016-2847-6
Volume15Issue:1Pages:137-144
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectOceanography
WOS Research AreaOceanography
WOS KeywordREAL-TIME PCR ; MESSENGER-RNA ; RT-PCR ; CHLAMYDOMONAS-REINHARDTII ; HOUSEKEEPING GENES ; NITRATE TRANSPORTER ; TRANSCRIPT LEVELS ; QUANTIFICATION ; NORMALIZATION ; TRIACYLGLYCEROLS
AbstractIsochrysis zhangjiangensis is a potential marine microalga for biodiesel production, which accumulates lipid under nitrogen limitation conditions, but the mechanism on molecular level is veiled. Quantitative real-time polymerase chain reaction (qPCR) provides the possibility to investigate the gene expression levels, and a valid reference for data normalization is an essential prerequisite for firing up the analysis. In this study, five housekeeping genes, actin (ACT), alpha-tubulin (TUA), beta-tubulin (TUB), ubiquitin (UBI), 18S rRNA (18S) and one target gene, diacylglycerol acyltransferase (DGAT), were used for determining the reference. By analyzing the stabilities based on calculation of the stability index and on operating the two types of software, geNorm and bestkeeper, it showed that the reference genes widely used in higher plant and microalgae, such as UBI, TUA and 18S, were not the most stable ones in nitrogen-stressed I. zhangjiangensis, and thus are not suitable for exploring the mRNA expression levels under these experimental conditions. Our results show that ACT together with TUB is the most feasible internal control for investigating gene expression under nitrogen-stressed conditions. Our findings will contribute not only to future qPCR studies of I. zhangjiangensis, but also to verification of comparative transcriptomics studies of the microalgae under similar conditions.
Language英语
WOS IDWOS:000371073400017
PublisherOCEAN UNIV CHINA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171277
Collection中国科学院大连化学物理研究所
Corresponding AuthorXue Song
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wu Shuang,Zhou Jiannan,Cao Xupeng,et al. Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2016,15(1):137-144.
APA Wu Shuang,Zhou Jiannan,Cao Xupeng,&Xue Song.(2016).Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition.JOURNAL OF OCEAN UNIVERSITY OF CHINA,15(1),137-144.
MLA Wu Shuang,et al."Determination of Internal Controls for Quantitative Gene Expression of Isochrysis zhangjiangensis at Nitrogen Stress Condition".JOURNAL OF OCEAN UNIVERSITY OF CHINA 15.1(2016):137-144.
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