DICP OpenIR
Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells
Lian, Jianchun1,2; Lv, Shijie3; Liu, Chang1,4; Liu, Yang1; Wang, Shujun1; Guo, Xin1; Nan, Feng5; Yu, Hua3; He, Xin2; Sun, Guangwei1; Ma, Xiaojun1
Source PublicationSTEM CELLS INTERNATIONAL
2016
ISSN1687-966X
DOI10.1155/2016/9291013
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectCell & Tissue Engineering
WOS Research AreaCell Biology
WOS KeywordBONE-MARROW ; GENE-EXPRESSION ; PROLIFERATION ; ACTIVATION ; PATHWAY ; ADIPOSE
AbstractBackground and Objective. It is important to guarantee the quality of stem cells. Serial passage is the main approach to expand stem cells. This study evaluated effects of serial passage on the biological characteristics of human umbilical cord Wharton's jelly-derived MSCs (WJ MSCs). Methods. Biological properties of WJ MSCs in the early (less than 10 passages, P10), middle (P11-20), and late (more than P20) phases including cell proliferation, cell cycle, phenotype, senescence, oncogene expression, stemness marker expression, and differentiation capacity were evaluated using flow cytometry, real-time PCR, immunocytofluorescence, and western blot. Results. It was found that there were no significant differences in cell proliferation, cell cycle, phenotype, and stemness marker expression in different phases. However, the expression of senescence-related gene, p21, and oncogene, c-Myc, was significantly upregulated in the late phase, which had close relations with the obviously increased cell senescence. Moreover, cardiac differentiation capability of WJ MSCs decreased whereas the propensity for neural differentiation increased significantly in the middle phase. Conclusions. This study reveals that WJ MSCs in the early and middle phases are relatively stable, and effect of serial passage on the lineage-specific differentiation should be considered carefully.
Language英语
WOS IDWOS:000369722900001
PublisherHINDAWI PUBLISHING CORP
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171363
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Yang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biotechnol, Dalian 116023, Peoples R China
2.Dalian Med Univ, Lab Med Coll, Dalian 116044, Peoples R China
3.Dalian Matern & Child Healthcare Hosp, Dalian 116033, Peoples R China
4.Uni Chinese Acad Sci, Beijing 100049, Peoples R China
5.Dalian Med Univ, Hosp 2, Dept Orthoped, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Lian, Jianchun,Lv, Shijie,Liu, Chang,et al. Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells[J]. STEM CELLS INTERNATIONAL,2016.
APA Lian, Jianchun.,Lv, Shijie.,Liu, Chang.,Liu, Yang.,Wang, Shujun.,...&Ma, Xiaojun.(2016).Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells.STEM CELLS INTERNATIONAL.
MLA Lian, Jianchun,et al."Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells".STEM CELLS INTERNATIONAL (2016).
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