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Magnetic nanoparticles coated with maltose-functionalized polyethyleneimine for highly efficient enrichment of N-glycopeptides
Li, Jinan1,2; Wang, Fangjun1; Wan, Hao1,2; Liu, Jing1,2; Liu, Zheyi1,2; Cheng, Kai1,2; Zou, Hanfa1
KeywordN-glycopeptides Enrichment Magnetic Nanoparticles Polyethyleneimine Click Chemistry Hydrophilic Interaction Chromatography
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2015-12-18
ISSN0021-9673
DOI10.1016/j.chroma.2015.11.044
Volume1425Pages:213-220
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; LECTIN AFFINITY-CHROMATOGRAPHY ; SELECTIVE ENRICHMENT ; LINKED GLYCOPEPTIDES ; MONOLITHIC CAPILLARY ; FACILE SYNTHESIS ; GLYCOPROTEIN ENRICHMENT ; TRACE GLYCOPROTEINS ; HYDRAZIDE CHEMISTRY ; MASS-SPECTROMETRY
AbstractHydrophilic interaction chromatography (HILIC) adsorbents have drawn increasing attention in recent years due to their high efficiency in N-glycopeptides enrichment. The hydrophilicity and binding capacity of HILIC adsorbents are crucial to the enrichment efficiency and mass spectrometry (MS) detection sensitivity of N-glycopeptides. Herein, magnetic nanoparticles coated with maltose-functionalized polyethyleneimine (Fe3O4-PEI-Maltose MNPs) were prepared by one-pot solvothermal reaction coupled with "click chemistry" and utilized for N-glycopeptides enrichment. Owing to the presence of hydrophilic and branched polyethyleneimine, the amount of immobilized disaccharide units was improved about four times. The N-glycopeptides capturing capacity was about 150 mg/g (IgG/MNPs) and the MS detection limitation as low as 115 fmol for IgG and 85% average enrichment recovery were feasibly achieved by using this hybrid magnetic adsorbent. Finally, 1237 unique N-glycosylation sites and 1567 unique N-glycopeptides from 684 N-glycoproteins were reliably characterized from 60 mu g protein sample extracted from mouse liver. Therefore, this maltose-functionalized polyethyleneimine coated adsorbent can play a promising role in highly efficient N-glycopeptides enrichment for glycoproteomic analyses of complex protein samples. (C) 2015 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000366770400024
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171531
Collection中国科学院大连化学物理研究所
Corresponding AuthorWang, Fangjun
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Being 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Jinan,Wang, Fangjun,Wan, Hao,et al. Magnetic nanoparticles coated with maltose-functionalized polyethyleneimine for highly efficient enrichment of N-glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1425:213-220.
APA Li, Jinan.,Wang, Fangjun.,Wan, Hao.,Liu, Jing.,Liu, Zheyi.,...&Zou, Hanfa.(2015).Magnetic nanoparticles coated with maltose-functionalized polyethyleneimine for highly efficient enrichment of N-glycopeptides.JOURNAL OF CHROMATOGRAPHY A,1425,213-220.
MLA Li, Jinan,et al."Magnetic nanoparticles coated with maltose-functionalized polyethyleneimine for highly efficient enrichment of N-glycopeptides".JOURNAL OF CHROMATOGRAPHY A 1425(2015):213-220.
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