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Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides
Zhang, Xihao1,2; Wang, Jiewen1,2; He, Xiwen1,2; Chen, Longxing1,2; Zhang, Yukui1,2,3
KeywordBoronate Magnetic Particles Distillation-precipitation Polymerization Glycoproteins Glycopeptides Enrichment
Source PublicationACS APPLIED MATERIALS & INTERFACES
2015-11-11
ISSN1944-8244
DOI10.1021/acsami.5b06445
Volume7Issue:44Pages:24576-24584
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS KeywordAFFINITY MONOLITHIC COLUMN ; DISTILLATION PRECIPITATION POLYMERIZATION ; SOLID-PHASE EXTRACTION ; N-LINKED GLYCOPROTEINS ; TOF MS ANALYSIS ; CIS-DIOL ; COMPOSITE MICROSPHERES ; NEUTRAL CONDITIONS ; MASS-SPECTROMETRY ; CAPTURE
AbstractBiomedical sciences, and in particular biomarker research, demand efficient glycoproteins enrichment platforms. In this work, we present a facile and time-saving method to synthesize phenylboronic acid and copolymer multifunctionalized magnetic nanoparticles (NPs) using a distillation precipitation polymerization (DPP) technique. The polymer shell is obtained through copolymerization of two monomers-affinity ligand 3-acrylaminophenylboronic acid (AAPBA) and a hydrophilic functional monomer. The resulting hydrophilic Fe3O4@P(AAPBA-co-monomer) NPs exhibit an enhanced binding capacity toward glycoproteins by an additional functional monomer complementary to the surface presentation of the target protein. The effects of monomer ratio of AAPBA to hydrophilic comonomers on the binding of glycoproteins are systematically investigated. The morphology, structure, and composition of all the synthesized microspheres are characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). The hydrophilic Fe3O4@P(AAPBA-co-monomer) microspheres show an excellent performance in the separation of glycoproteins with high binding capacity; And strong magnetic response allows them to be easily separated from solution in the presence of an external magnetic field. Moreover, both synthetic Fe(3)O(4)gP(AAPBA) and copolymeric NPs show good adsorption to glycoproteins in physiological conditions (pH 7.4). The Fe3O4@P(AAPBA-co-monomer) NPs are successfully utilized to selectively capture and identify the low-abundance glycopeptides from the tryptic digest of horseradish peroxidase (HRP). In addition, the selective isolation and enrichment of glycoproteins from the egg white samples at physiological condition is obtained by Fe3O4@P(AAPBA-co-monomer) NPs as adsorbents.
Language英语
WOS IDWOS:000364726400022
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171613
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen, Longxing
Affiliation1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Coll Chem,Res Ctr Anal Sci, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xihao,Wang, Jiewen,He, Xiwen,et al. Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(44):24576-24584.
APA Zhang, Xihao,Wang, Jiewen,He, Xiwen,Chen, Longxing,&Zhang, Yukui.(2015).Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides.ACS APPLIED MATERIALS & INTERFACES,7(44),24576-24584.
MLA Zhang, Xihao,et al."Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides".ACS APPLIED MATERIALS & INTERFACES 7.44(2015):24576-24584.
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