DICP OpenIR
Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation
Huang, Chuncui1,5; Yan, Jingyu2; Zhan, Lingpeng3,6; Zhao, Min1,3; Zhou, Jinyu1,3; Gao, Huanyu1; Xie, Wenchun1,5; Li, Yan1,3; Chai, Wengang4
Corresponding AuthorLi, Yan(yanli@ibp.ac.cn)
KeywordNegative-ion matrix-assisted laser desorption/ionization tandem mass spectrometry Laser-induced dissociation Linkage analysis Gluco-oligosaccharides Blood-group related antigens Typing
Source PublicationANALYTICA CHIMICA ACTA
2019-09-13
ISSN0003-2670
DOI10.1016/j.aca.2019.04.067
Volume1071Pages:25-35
Funding ProjectNational Natural Science Foundation of China[31600650] ; National Natural Science Foundation of China[31671369] ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center[22-FY18-821]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordUNDERIVATIZED OLIGOSACCHARIDES ; SIALYLATED OLIGOSACCHARIDES ; BRANCHING PATTERN ; POSTSOURCE DECAY ; ANIONIC ADDUCTS ; TOF MS ; PART 1 ; MATRIX ; DESORPTION/IONIZATION ; FRAGMENTATION
AbstractMass spectrometry (MS) has become the primary method for high-sensitivity structural determination of oligosaccharides. Fragmentation in the negative-ion MS can provide a wealth of structural information and these can be used for sequence determination. However, although negative-ion MS of neutral oligosaccharide using the deprotonated molecule [M-H](-) as the precursor has been very successful for electrospray ionization (ESI), it has only limited success for matrix-assisted laser desorption/ionization (MALDI). In the present study, the features of negative-ion MALDI primary spectra were investigated in detail and the product-ion spectra using [M-H](-) and [M+Cl](-) as the precursors were carefully compared. The formation of [M-H](- )was the main difficulty for MALDI while [M+Cl](-) was proved to be useful as alternative precursor anion for MALDI-MS/MS to produce similar fragmentation for sequencing of neutral oligosaccharides. N-(1-naphthyl)ethylenediamine dihydrochloride was then used as both the matrix and the Cl- dopant to evaluate the extent of structural information that can be obtained by negative-ion fragmentation from [M+Cl](-) using laser-induced dissociation (LID)-MS/MS for linkage assignment of gluco-oligosaccharides and for typing of blood-group ABO(H) and Lewis antigens on either type 1 or type 2 backbone-chains. (C) 2019 Elsevier B.V. All rights reserved.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center ; March of Dimes (Arlington, VA, U.S.A) Prematurity Research Center
WOS IDWOS:000468581400004
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171872
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi, Yan
Affiliation1.Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Imperial Coll London, Glycosci Lab, Fac Med, Hammersmith Campus, London W12 0NN, England
5.GuangDong Biohealtech Adv, Foshan 528315, Peoples R China
6.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Huang, Chuncui,Yan, Jingyu,Zhan, Lingpeng,et al. Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation[J]. ANALYTICA CHIMICA ACTA,2019,1071:25-35.
APA Huang, Chuncui.,Yan, Jingyu.,Zhan, Lingpeng.,Zhao, Min.,Zhou, Jinyu.,...&Chai, Wengang.(2019).Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation.ANALYTICA CHIMICA ACTA,1071,25-35.
MLA Huang, Chuncui,et al."Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation".ANALYTICA CHIMICA ACTA 1071(2019):25-35.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Chuncui]'s Articles
[Yan, Jingyu]'s Articles
[Zhan, Lingpeng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Chuncui]'s Articles
[Yan, Jingyu]'s Articles
[Zhan, Lingpeng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Chuncui]'s Articles
[Yan, Jingyu]'s Articles
[Zhan, Lingpeng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.