DICP OpenIR
A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy
Qi, Qingkai1; Chi, Weijie2; Li, Yuanyuan3; Qiao, Qinglong1; Chen, Jie1; Miao, Lu1; Zhang, Yi1; Li, Jin1; Ji, Wei3; Xu, Tao3; Liu, Xiaogang2; Yoon, Juyoung4; Xu, Zhaochao1
Corresponding AuthorXu, Tao(xutao@ibp.ac.cn) ; Liu, Xiaogang(xiaogang_liu@sutd.edu.sg) ; Yoon, Juyoung(jyoon@ewha.ac.kr) ; Xu, Zhaochao(zcxu@dicp.ac.cn)
Source PublicationCHEMICAL SCIENCE
2019-05-14
ISSN2041-6520
DOI10.1039/c9sc01284b
Volume10Issue:18Pages:4914-4922
Funding ProjectNational Natural Science Foundation of China[21708039] ; National Natural Science Foundation of China[21502189] ; National Natural Science Foundation of China[21878286] ; CPSF[2017M611278] ; CPSF[2018T110234] ; DICP[DMTO201603] ; DICP[TMSR201601] ; DICP[2015YB07] ; SUTD[T1SRCI17126] ; SUTD[IDD21700101] ; SUTD[IDG31800104] ; National Research Foundation of Korea (NRF)[2012R1A3A2048814]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF)
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordFLUORESCENCE NANOSCOPY ; DOMAINS ; APOPTOSIS ; CELLS ; DIARYLETHENES ; VISUALIZATION ; PHOTOCHROMISM
AbstractRhodamine spirolactam based photoswitches have been extensively applied in super-resolution single-molecule localization microscopy (SMLM). However, the ring-opening reactions of spirolactams are cross-sensitive to acid, limiting their photoswitch use to neutral pH conditions. In addition, the ring-closing reactions of spirolactams are environment-sensitive and slow (up to hours), virtually making rhodamine spirolactams caged fluorescent dyes instead of reversible photoswitches in SMLM. Herein, by introducing hydrogen bonds to stabilize spirolactams, we report a series of acid-resistant rhodamine spirolactams with accelerated ring-closing reactions from fluorescent xanthyliums to non-fluorescent spirolactams, endowing them with good photoswitchable properties even in acidic environments. By further substitution of 6-phenylethynyl naphthalimide on the spirolactam, we shifted the photoactivation wavelength into the visible region (>400 nm). Subsequently, we have successfully applied these dyes in labeling and imaging the cell surface of Bacillus subtilis at pH 4.5 using SMLM.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF)
WOS IDWOS:000468818700020
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171928
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu, Tao; Liu, Xiaogang; Yoon, Juyoung; Xu, Zhaochao
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Singapore Univ Technol & Design, 8 Somapah Rd, Singapore 487372, Singapore
3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
4.Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
Recommended Citation
GB/T 7714
Qi, Qingkai,Chi, Weijie,Li, Yuanyuan,et al. A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy[J]. CHEMICAL SCIENCE,2019,10(18):4914-4922.
APA Qi, Qingkai.,Chi, Weijie.,Li, Yuanyuan.,Qiao, Qinglong.,Chen, Jie.,...&Xu, Zhaochao.(2019).A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy.CHEMICAL SCIENCE,10(18),4914-4922.
MLA Qi, Qingkai,et al."A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy".CHEMICAL SCIENCE 10.18(2019):4914-4922.
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