DICP OpenIR
Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori
Liu, Dian1,2; Jin, Hongli1; Wang, Jixia1; Zhou, Han1; Liu, Yanfang1; Feng, Jiatao1; Liang, Xinmiao1
Corresponding AuthorLiu, Yanfang(liuyanfang@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
KeywordCaulis Polygoni Multiflori Polar compounds Polar-copolymerized reversed-phase chromatography Zwitterionic hydrophilic interaction chromatography
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2019-06-15
ISSN1570-0232
DOI10.1016/j.jchromb.2019.04.039
Volume1118Pages:70-77
Funding ProjectState Key Program of National Natural Science of China[U1508221] ; National Science Foundation of China[31670374] ; National Science Foundation of China[81403100] ; Innovation Program of science and research from DICP, CAS[DICP TMSR201601] ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine
Funding OrganizationState Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; BASIC COMPOUNDS ; MASS-SPECTROMETRY ; C18 COLUMN ; SEPARATION ; ALKALOIDS ; ONLINE ; COMPONENTS ; SYSTEM
AbstractPolar compounds are abundant in the water decoction of Traditional Chinese Medicines (TCMs) and possess important biological activities. However, purifying these compounds has faced great difficulties largely due to poor retention and insufficient selectivity. To solve this problem, an offline orthogonal 2-D RPLC coupled with hydrophilic interaction chromatography (HILIC) method was developed to achieve purification of polar compounds from Caulis Polygoni Multiflori. A polar-copolymerized XAqua C18 column and a zwitterionic Click XIon column exhibited satisfactory retention and separation for polar compounds. Therefore, they were adopted to construct the offline 2-D LC system. Furthermore, the method presented a high orthogonality, which was calculated to be 72%. The)(Aqua C18 column was used in the first dimension to fractionate the 5.1 g polar fraction with a recovery of 85% within 10 h. In the second dimension, three representative fractions were purified using the Click XIon column. Finally, three compounds with purity higher than 95% were identified for the first time from this plant. This offline 2-D RPLC/HILIC method was shown to be an effective approach to purifying polar compounds from Caulis Polygoni Multiflori.
Language英语
Funding OrganizationState Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine
WOS IDWOS:000469153600010
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/171946
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiu, Yanfang; Liang, Xinmiao
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Dian,Jin, Hongli,Wang, Jixia,et al. Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1118:70-77.
APA Liu, Dian.,Jin, Hongli.,Wang, Jixia.,Zhou, Han.,Liu, Yanfang.,...&Liang, Xinmiao.(2019).Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1118,70-77.
MLA Liu, Dian,et al."Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1118(2019):70-77.
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