DICP OpenIR
Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy
Hu, Yechen1,2; Weng, Yejing1,2; Jiang, Bo1; Li, Xiao1; Zhang, Xiaodan1; Zhao, Baofeng1; Wu, Qiong1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
Corresponding AuthorZhang, Lihua(lihuazhang@dicp.ac.cn)
Keywordphosphorylated lysine retention time difference dimethyl labeling N-phosphorylation proteome LC-MS/MS
Source PublicationSCIENCE CHINA-CHEMISTRY
2019-06-01
ISSN1674-7291
DOI10.1007/s11426-018-9433-3
Volume62Issue:6Pages:708-712
Funding ProjectNational Key Research and Development Program of China[2017YFA0505003] ; National Key Research and Development Program of China[2016YFA0501401] ; National Natural Science Foundation of China[21505133] ; National Natural Science Foundation of China[21725506] ; National Natural Science Foundation of China[91543201] ; CAS Key Project in Frontier Science[QYZDY-SSW-SLH017] ; DICP, Chinese Academy of Sciences[DICP TMSR201601]
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordLABILE HISTONE PHOSPHATES ; HISTIDINE PHOSPHORYLATION ; ARGININE PHOSPHORYLATION ; ESCHERICHIA-COLI ; CHEMICAL BIOLOGY ; PROTEIN-KINASES ; PHOSPHOHISTIDINE ; PHOSPHOARGININE ; RAT ; PHOSPHOPROTEOME
AbstractProtein phosphorylation plays essential roles in various biological procedures. Despite the well-established enrichment strategies for O-phosphoproteomics, the intrinsic acid lability of N-P phosphoramidate bond (phosphorylation of histidine, arginine and lysine) has impaired the progress of N-phosphoproteomics. Herein, we reported a retention time difference combining dimethyl labeling (ReDD) strategy for the isolation and identification of phosphorylated lysine (pLys) peptides. By such a method, pLys peptide could be isolated under 100000-fold interference of non-phosphorylated peptides. Furthermore, ReDD strategy was applied to map pLys sites from E. coli samples, leading to the identification of 11 pLys sites, among which K26p that originating from autonomous glycyl radical cofactor was validated both in mass spectrometry and HPLC co-elution experiments. Furthermore, 112 pLys sites from 100 proteins were identified in HeLa cells. All these results demonstrate that ReDD could provide a first glimpse into Lys phosphorylation, and could be an important step toward the global perspective on protein phosphorylation.
Language英语
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences
WOS IDWOS:000470762900008
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/172037
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Hu, Yechen,Weng, Yejing,Jiang, Bo,et al. Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy[J]. SCIENCE CHINA-CHEMISTRY,2019,62(6):708-712.
APA Hu, Yechen.,Weng, Yejing.,Jiang, Bo.,Li, Xiao.,Zhang, Xiaodan.,...&Zhang, Yukui.(2019).Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy.SCIENCE CHINA-CHEMISTRY,62(6),708-712.
MLA Hu, Yechen,et al."Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy".SCIENCE CHINA-CHEMISTRY 62.6(2019):708-712.
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