DICP OpenIR
Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system
Hou Dandan1; Yu Weiting2; Zhang Demeng3; Zhao Lili3; Liu Xiudong1; Ma Xiaojun4
Corresponding AuthorYu Weiting(yuwt@dicp.ac.cn) ; Liu Xiudong(liuxiudong@dlu.edu.cn)
Keywordalginate-chitosan (AC) microcapsules immobilization biocatalysis aqueous-solvent biphasic system cell growth
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
2019-05-01
ISSN2096-5508
DOI10.1007/s00343-019-8126-9
Volume37Issue:3Pages:863-870
Funding ProjectNational Natural Science Foundation of China[21276033] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1707] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1711] ; Liaoning Provincial BaiQianWan Talents Program[2017-6]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program
WOS SubjectLimnology ; Oceanography
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS KeywordBIOCATALYSIS ; MICROENCAPSULATION ; VIABILITY
AbstractImmobilization biocatalysis is a potential technology to improve the activity and stability of biocatalysts in nonaqueous systems for efficient industrial production. Alginate-chitosan (AC) microcapsules were prepared as immobilization carriers by emulsification-internal gelation and complexation reaction, and their contribution on facilitating the growth and metabolism of yeast cells were testified successfully in culture medium-solvent biphasic systems. The cell growth in AC microcapsules is superior to that in alginate beads, and the cells in both immobilization carriers maintain much higher activity than free cells, which demonstrates AC microcapsules can confer yeast cells the ability to resist the adverse effect of solvent. Moreover, the performance of AC microcapsules in biphasic systems could be improved by adjusting the formation of outer polyelectrolyte complex (PEC) membrane to promote the cell growth and metabolic ability under the balance of resisting solvent toxicity and permitting substrate diffusion. Therefore, these findings are quite valuable for applying AC microcapsules as novel immobilization carriers to realize the biotransformation of value-added products in aqueous-solvent biphasic systems.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program
WOS IDWOS:000469401600010
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/172080
Collection中国科学院大连化学物理研究所
Corresponding AuthorYu Weiting; Liu Xiudong
Affiliation1.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
2.Dalian Univ, Affiliated Zhongshan Hosp, Dalian 116001, Peoples R China
3.Qingdao Brightmoon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Shandong, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Hou Dandan,Yu Weiting,Zhang Demeng,et al. Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(3):863-870.
APA Hou Dandan,Yu Weiting,Zhang Demeng,Zhao Lili,Liu Xiudong,&Ma Xiaojun.(2019).Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(3),863-870.
MLA Hou Dandan,et al."Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.3(2019):863-870.
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