DICP OpenIR
Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog
Wang Zhi-Chao1,2; Liu Jing1; Piao Hai-Long1,2
Corresponding AuthorPiao Hai-Long(hpiao@dicp.ac.cn)
KeywordCapillary electrophoresis-mass spectrometry Cell metabolomics Phosphatase and tensin homolog Metabolic reprogramming Prostate cancer
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2019
ISSN0253-3820
DOI10.19756/j.issn.0253-3820.181600
Volume47Issue:1Pages:59-66
Funding ProjectNational Natural Science Foundation of China[81672440] ; National Natural Science Foundation of China[81702771] ; Innovation Program of Science and Research from the DICP, CAS ; DICP[TMSR201601]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordCANCER ; PTEN ; BREAST ; VULNERABILITY ; HYPOXIA
AbstractProstate cancer is the most frequently occurred cancer in males. Phosphatase and tensin homolog ( PTEN ) deficiency often occurs in prostate cancer and induces metabolic reprogramming. Metabolic vulnerabilities induced by PTEN deficiency may provide therapeutic targets for cancer therapy. Here, capillary electrophoresis-mass spectrometry ( CE-MS ) based metabolomics analysis was used for analyzing metabolic changes induced by PTEN deficiency in prostate cancer cell DU145 and normal prostate cell RWPE1. 200 and 214 metabolites were detected, and 28 and 37 differential metabolites were authenticated in PTEN knock-downed DU145 and RWPE1 cells compared to their controls , respectively. Threonic acid levels increased , while isobutyrylcarnitine , adenosine diphosphate , N-glycolylneuraminic acid, Asp hypotaurine levels decreased after PTEN silencing in both cell lines. The specific metabolites changes in DU145 after PTEN silencing were L-2-HG, glycerophosphocholine , thiamine, the ratio of GSH to GSSG, and all of them were increased. These metabolites can promote tumor proliferation, metastasis, and resistance to chemotherapy. Creatinine, carnosine and N-acetylneuraminic acid , which had been reported to be biomarkers of cancer diagnosis and prognosis, were regulated by PTEN deficiency. Metabolites changes induced by PTEN deficiency only or combined effect of PTEN deficiency and other cancer-related genes were identified.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP
WOS IDWOS:000470997900007
PublisherELSEVIER SCIENCE INC
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/172164
Collection中国科学院大连化学物理研究所
Corresponding AuthorPiao Hai-Long
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang Zhi-Chao,Liu Jing,Piao Hai-Long. Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2019,47(1):59-66.
APA Wang Zhi-Chao,Liu Jing,&Piao Hai-Long.(2019).Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,47(1),59-66.
MLA Wang Zhi-Chao,et al."Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 47.1(2019):59-66.
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