DICP OpenIR
An overview on enrichment methods for cell surface proteome profiling
Li, Yanan1,2; Qin, Hongqiang1; Ye, Mingliang1
Corresponding AuthorYe, Mingliang(mingliang@dicp.ac.cn)
Keywordcell surface proteins enrichment methods plasma membrane proteins proteomics
Source PublicationJOURNAL OF SEPARATION SCIENCE
2019-09-30
ISSN1615-9306
DOI10.1002/jssc.201900700
Pages21
Funding ProjectNational Natural Science Foundation of China[81430072] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[91753105] ; China State Key Basic Research Program[2016YFA0501402] ; China State Key Basic Research Program[2017YFA0505004] ; China State Key Basic Research Program[2018YFC0910302] ; innovation program of science and research from the DICP[DICP TMSR201601] ; innovation program of science and research from the DICP[DICP& QIBEBT UN201802] ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars[21525524]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPLASMA-MEMBRANE PROTEINS ; FREE-FLOW ELECTROPHORESIS ; ENZYME-MEDIATED ACTIVATION ; EMBRYONIC STEM-CELLS ; MASS-SPECTROMETRY ; LECTIN AFFINITY ; IN-VIVO ; CLICK CHEMISTRY ; N-GLYCOPROTEOME ; LIVING CELLS
AbstractCell surface proteins are essential for many important biological processes, including cell-cell interactions, signal transduction, and molecular transportation. With the characteristics of low abundance, high hydrophobicity, and high heterogeneity, it is difficult to get a comprehensive view of cell surface proteome by direct analysis. Thus, it is important to selectively enrich the cell surface proteins before liquid chromatography with mass spectrometry analysis. In recent years, a variety of enrichment methods have been developed. Based on the separation mechanism, these methods could be mainly classified into three types. The first type is based on their difference in the physicochemical property, such as size, density, charge, and hydrophobicity. The second one is based on the bimolecular affinity interaction with lectin or antibody. And the third type is based on the chemical covalent coupling to free side groups of surface-exposed proteins or carbohydrate chains, such as primary amines, carboxyl groups, glycan side chains. In addition, metabolic labeling and enzymatic reaction-based methods have also been employed to selectively isolate cell surface proteins. In this review, we will provide a comprehensive overview of the enrichment methods for cell surface proteome profiling.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars
WOS IDWOS:000488151300001
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/172488
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe, Mingliang
Affiliation1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Yanan,Qin, Hongqiang,Ye, Mingliang. An overview on enrichment methods for cell surface proteome profiling[J]. JOURNAL OF SEPARATION SCIENCE,2019:21.
APA Li, Yanan,Qin, Hongqiang,&Ye, Mingliang.(2019).An overview on enrichment methods for cell surface proteome profiling.JOURNAL OF SEPARATION SCIENCE,21.
MLA Li, Yanan,et al."An overview on enrichment methods for cell surface proteome profiling".JOURNAL OF SEPARATION SCIENCE (2019):21.
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