DICP OpenIR
A microfluidic strategy to fabricate ultra-thin polyelectrolyte hollow microfibers as 3D cellular carriers
Liu, Hui1; Wang, Yaqing1,4; Chen, Wenwen1,4; Yu, Yue1; Jiang, Lei1; Qin, Jianhua1,2,3,4
Corresponding AuthorQin, Jianhua(jhqin@dicp.ac.cn)
KeywordMicrofluidics Alginate Chitosan Ultra-thin polyelectrolyte microfibers Cell culture
Source PublicationMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
2019-11-01
ISSN0928-4931
DOI10.1016/j.msec.2019.04.084
Volume104Pages:10
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA16020900] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB29050301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB32030200] ; National Natural Science Foundation of China[91543121] ; National Natural Science Foundation of China[81573394] ; National Natural Science Foundation of China[31671038] ; National Natural Science Foundation of China[31600784] ; National Key Research and Development Program of China[2017YFB0405400] ; Key Program of the Chinese Academy of Sciences[KFZD-SW-213] ; Innovation Program of Science and Research from the DICP, CAS[DICP TMSR201601]
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS
WOS SubjectMaterials Science, Biomaterials
WOS Research AreaMaterials Science
WOS KeywordHYDROGEL MICROFIBERS ; LADEN MICROFIBERS ; FIBERS ; COMPOSITE ; MICROCAPSULES ; CHITOSAN ; MATRIX
AbstractMicrofluidics-based microfibers have been widely used as bottom-up scaffolds for tissue engineering applications. Different forms of microfibers with certain thickness of shell have been developed during the past decade. Ultra-thin microfiber, as a special and promising carrier of cells, was less explored. In this work, by using the interfacial ionic interaction between sodium alginate (NaA) and chitosan (CS), a novel ultra-thin polyelectrolyte hollow microfiber with the diameter of similar to 200 mu m and the shell thickness of 1.3 +/- 0.3 mu m was fabricated via a microfluidic device for liver tissue engineering. The fluorescence of FITC labeled CS confirmed the inner CS layer of the fabricated microfiber and the SEM results illustrated its ultra-thin characteristic. Although there are only two layers in the ultra-thin polyelectrolyte hollow microfiber, the following cells encapsulation experiments indicated that it could bear cells loading and the hollow space of the microfibers could encapsulate sufficient number of cells for tissue engineering applications. The presence of inner CS layer in the microfiber promoted cell adhesion and ultra-thin shell characteristic facilitated the exchange of nutrient substance and O-2 and thus promoted cell proliferation. HepG2 cells encapsulated in the microfibers maintained favorable viability, proliferation ability and hepatic specific functions during 10 days' culture. These results suggest that the established polyelectrolyte microfibers hold great potential applications in the field of liver tissue engineering. We believe this work will lead to the development of innovative methodologies and materials for both cell culture and biomedical application.
Language英语
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS
WOS IDWOS:000487569300130
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/172683
Collection中国科学院大连化学物理研究所
Corresponding AuthorQin, Jianhua
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China
3.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hui,Wang, Yaqing,Chen, Wenwen,et al. A microfluidic strategy to fabricate ultra-thin polyelectrolyte hollow microfibers as 3D cellular carriers[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2019,104:10.
APA Liu, Hui,Wang, Yaqing,Chen, Wenwen,Yu, Yue,Jiang, Lei,&Qin, Jianhua.(2019).A microfluidic strategy to fabricate ultra-thin polyelectrolyte hollow microfibers as 3D cellular carriers.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,104,10.
MLA Liu, Hui,et al."A microfluidic strategy to fabricate ultra-thin polyelectrolyte hollow microfibers as 3D cellular carriers".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 104(2019):10.
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