DICP OpenIR
Dual-Functional Titanium(IV) Immobilized Metal Affinity Chromatography Approach for Enabling Large-Scale Profiling of Protein Mannose-6-Phosphate Glycosylation and Revealing Its Predominant Substrates
Huang, Junfeng1; Dong, Jing4; Shi, Xudong2; Chen, Zhengwei3; Cui, Yusi3; Liu, Xiaoyan4; Ye, Mingliang4; Li, Lingjun1,3,5
Corresponding AuthorYe, Mingliang(mingliang@dicp.ac.cn) ; Li, Lingjun(lingjun.li@wisc.edu)
Source PublicationANALYTICAL CHEMISTRY
2019-09-17
ISSN0003-2700
DOI10.1021/acs.analchem.9b01698
Volume91Issue:18Pages:11589-11597
Funding ProjectNational Key R&D Program of China[2016YFA0501402] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[21525524] ; National Institutes of Health[U01CA231081] ; National Institutes of Health[RF1AG052324] ; National Institutes of Health[ROI DK071801] ; NIH Shared Instrument Grant[NIH-NCRR S10RR029531] ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordLYSOSOMAL ACID LIPASE ; MANNOSE 6-PHOSPHATE ; PHOSPHOPEPTIDE ENRICHMENT ; IDENTIFICATION ; PHOSPHORYLATION ; GLYCOPROTEOMICS ; LIVER ; PROTEOMICS ; RECEPTOR ; ENZYMES
AbstractMannose-6-phosphate (M6P) glycosylation is an important post-translational modification (PTM) and plays a crucial role in transferring lysosomal hydrolases to lysosome, and is involved in several other biological processes. Aberrant M6P modifications have been implicated in lysosomal storage diseases and numerous other disorders including Alzheimer's disease and cancer. Research on profiling of intact M6P glycopeptides remains challenging due to its extremely low stoichiometry. Here we propose a dual-mode affinity approach to enrich M6P glycopeptides by dual-functional titanium(IV) immobilized metal affinity chromatography [Ti(IV)-IMAC] materials. In combination with state-of-the-art mass spectrometry and database search engine, we profiled 237 intact M6P glycopeptides corresponding to 81 M6P glycoproteins in five types of tissues in mouse, representing the first large-scale profiling of M6P glycosylation in mouse samples. The analysis of M6P glycoforms revealed the predominant glycan substrates of this PTM. Gene ontology analysis showed that overrepresented M6P glycoproteins were lysosomal-associated proteins. However, there were still substantial M6P glycoproteins that possessed different subcellular locations and molecular functions. Deep mining of their roles implicated in lysosomal and nonlysosomal function can provide new insights into functional roles of this important yet poorly studied modification.
Language英语
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health ; National Institutes of Health ; NIH Shared Instrument Grant ; NIH Shared Instrument Grant ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; University of Wisconsin Madison, Office of the Vice Chancellor for Research and Graduate Education ; Wisconsin Alumni Research Foundation ; Wisconsin Alumni Research Foundation ; University of Wisconsin-Madison School of Pharmacy ; University of Wisconsin-Madison School of Pharmacy
WOS IDWOS:000487156900017
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/172772
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe, Mingliang; Li, Lingjun
Affiliation1.Univ Wisconsin, Sch Pharm, Madison, WI 53705 USA
2.Univ Wisconsin, Dept Surg, Madison, WI 53705 USA
3.Univ Wisconsin, Dept Chem, Madison, WI 53705 USA
4.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
5.Tianjin Univ, Sch Life Sci, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Huang, Junfeng,Dong, Jing,Shi, Xudong,et al. Dual-Functional Titanium(IV) Immobilized Metal Affinity Chromatography Approach for Enabling Large-Scale Profiling of Protein Mannose-6-Phosphate Glycosylation and Revealing Its Predominant Substrates[J]. ANALYTICAL CHEMISTRY,2019,91(18):11589-11597.
APA Huang, Junfeng.,Dong, Jing.,Shi, Xudong.,Chen, Zhengwei.,Cui, Yusi.,...&Li, Lingjun.(2019).Dual-Functional Titanium(IV) Immobilized Metal Affinity Chromatography Approach for Enabling Large-Scale Profiling of Protein Mannose-6-Phosphate Glycosylation and Revealing Its Predominant Substrates.ANALYTICAL CHEMISTRY,91(18),11589-11597.
MLA Huang, Junfeng,et al."Dual-Functional Titanium(IV) Immobilized Metal Affinity Chromatography Approach for Enabling Large-Scale Profiling of Protein Mannose-6-Phosphate Glycosylation and Revealing Its Predominant Substrates".ANALYTICAL CHEMISTRY 91.18(2019):11589-11597.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Junfeng]'s Articles
[Dong, Jing]'s Articles
[Shi, Xudong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Junfeng]'s Articles
[Dong, Jing]'s Articles
[Shi, Xudong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Junfeng]'s Articles
[Dong, Jing]'s Articles
[Shi, Xudong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.