DICP OpenIR
The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite
Niu, Huan1,2; Li, Xin1,2; Peng, Jiaxi1,2; Zhang, Hongyan1,2; Zhao, Xingyun1,2; Zhou, Xiaoyu1,2; Yu, Dongping1,2; Liu, Xinyu1; Wu, Ren'an1
Corresponding AuthorWu, Ren'an(wurenan@dicp.ac.cn)
Source PublicationANALYST
2019-09-07
ISSN0003-2654
DOI10.1039/c9an01119f
Volume144Issue:17Pages:5261-5270
Funding ProjectNational Natural Science Foundation of China[21675156] ; National Natural Science Foundation of China[21375125] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201503] ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics[DICP TMSR201601]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSELECTIVE ENRICHMENT ; FACILE PREPARATION ; HIGH-SENSITIVITY ; HIGH-THROUGHPUT ; CARBON ; GLYCOSYLATION ; GLYCOMICS ; CANCER ; OLIGOSACCHARIDES ; GLYCOPROTEOMICS
AbstractIn this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation. In tailoring the large surface area (ca. 2213 m(2) g(-1)) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference. For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng mu L-1 with the identification of 13 N-linked glycans (S/N > 10). When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities. From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison. In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics
WOS IDWOS:000483593000022
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/173310
Collection中国科学院大连化学物理研究所
Corresponding AuthorWu, Ren'an
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Niu, Huan,Li, Xin,Peng, Jiaxi,et al. The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite[J]. ANALYST,2019,144(17):5261-5270.
APA Niu, Huan.,Li, Xin.,Peng, Jiaxi.,Zhang, Hongyan.,Zhao, Xingyun.,...&Wu, Ren'an.(2019).The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.ANALYST,144(17),5261-5270.
MLA Niu, Huan,et al."The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite".ANALYST 144.17(2019):5261-5270.
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