DICP OpenIR
Linking bioenergetic function of mitochondria to tissue-specific molecular fingerprints
Kappler, Lisa1; Hoene, Miriam1; Hu, Chunxiu2; von Toerne, Christine3; Li, Jia1,4; Bleher, Daniel1; Hoffmann, Christoph1; Boehm, Anja5,6; Kollipara, Laxmikanth7; Zischka, Hans8,9; Koenigsrainer, Alfred10,11,12; Haering, Hans-Ulrich1,5,6; Peter, Andreas1,5,6; Xu, Guowang2; Sickmann, Albert7,13,14; Hauck, Stefanie M.3,6; Weigert, Cora1,5,6; Lehmann, Rainer1,5,6
Corresponding AuthorLehmann, Rainer(rainer.lehmann@med.uni-tuebingen.de)
Keywordcardiolipins liver mitochondria multi-omics muscle
Source PublicationAMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
2019-08-01
ISSN0193-1849
DOI10.1152/ajpendo.00088.2019
Volume317Issue:2Pages:E374-E387
Funding ProjectAlexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF)[01GI0925] ; National Key Research and Development Program of China[2017YFC0906900] ; National Natural Science Foundation of China[21874130] ; National Natural Science Foundation of China[21435006] ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF
Funding OrganizationAlexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF ; Alexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF ; Alexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF ; Alexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF
WOS SubjectEndocrinology & Metabolism ; Physiology
WOS Research AreaEndocrinology & Metabolism ; Physiology
WOS KeywordSKELETAL-MUSCLE ; RESPIRATORY-CHAIN ; OXIDATIVE-PHOSPHORYLATION ; SUPERCOMPLEX FORMATION ; LINOLEIC-ACID ; CARDIOLIPIN ; LIVER ; PHOSPHOLIPIDS ; GLUCONEOGENESIS ; DYSFUNCTION
AbstractMitochondria are dynamic organelles with diverse functions in tissues such as liver and skeletal muscle. To unravel the mitochondrial contribution to tissue-specific physiology, we performed a systematic comparison of the mitochondrial proteome and lipidome of mice and assessed the consequences hereof for respiration. Liver and skeletal muscle mitochondrial protein composition was studied by data-independent ultra-high-performance (UHP)LC-MS/MS-proteomics, and lipid profiles were compared by UIIPLC-MS/MS lipidomics. Mitochondrial function was investigated by high-resolution respirometry in samples from mice and humans. Enzymes of pyruvate oxidation as well as several subunits of complex I, III, and ATP synthase were more abundant in muscle mitochondria. Muscle mitochondria were enriched in cardiolipins associated with higher oxidative phosphorylation capacity and flexibility, in particular CL(18:2)(4) and 22:6-containing cardiolipins. In contrast, protein equipment of liver mitochondria indicated a shuttling of complex I substrates toward gluconeogenesis and ketogenesis and a higher preference for electron transfer via the flavoprotein quinone oxidoreductase pathway. Concordantly, muscle and liver mitochondria showed distinct respiratory substrate preferences. Muscle respired significantly more on the complex I substrates pyruvate and glutamate, whereas in liver maximal respiration was supported by complex II substrate succinate. This was a consistent finding in mouse liver and skeletal muscle mitochondria and human samples. Muscle mitochondria are tailored to produce ATP with a high capacity for complex I-linked substrates. Liver mitochondria are more connected to biosynthetic pathways, preferring fatty acids and succinate for oxidation. The physiologic diversity of mitochondria may help to understand tissue-specific disease pathologies and to develop therapies targeting mitochondrial function.
Language英语
Funding OrganizationAlexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF ; Alexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF ; Alexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF ; Alexander von Humboldt Foundation ; Alexander von Humboldt Foundation ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; Regierende Burgermeister von Berlin - inkl. Wissenschaft und Forschung ; BMBF ; BMBF
WOS IDWOS:000481599900011
PublisherAMER PHYSIOLOGICAL SOC
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/173524
Collection中国科学院大连化学物理研究所
Corresponding AuthorLehmann, Rainer
Affiliation1.Univ Hosp Tuebingen, Inst Clin Chem & Pathobiochem, Dept Diagnost Lab Med, Hoppe Seyler Str 3, D-72076 Tubingen, Germany
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Helmholtz Ctr Munich, Res Unit Prot Sci, Munich, Germany
4.Chinese Acad Agr Sci, Tea Res Inst, Minist Agr, Key Lab Tea Biol & Resources Utilizat, Hangzhou, Zhejiang, Peoples R China
5.Univ Tubingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, Tubingen, Germany
6.German Ctr Diabet Res, Tubingen, Germany
7.Leibniz Inst Analyt Wissensch ISAS, Dortmund, Germany
8.German Res Ctr Environm Hlth GmbH, Helmholtz Ctr Munich, Inst Mol Toxicol & Pharmacol, Neuherberg, Germany
9.Tech Univ Munich, Inst Toxicol & Environm Hyg, Munich, Germany
10.Univ Hosp Tuebingen, Dept Gen Visceral & Transplant Surg, Tubingen, Germany
11.German Canc Consortium DKTK, Tubingen, Germany
12.German Canc Res Ctr DKFZ Partner Site, Tubingen, Germany
13.Ruhr Univ Bochum, Med Proteom Ctr, Bochum, Germany
14.Univ Aberdeen, Coll Phys Sci, Dept Chem, Aberdeen, Scotland
Recommended Citation
GB/T 7714
Kappler, Lisa,Hoene, Miriam,Hu, Chunxiu,et al. Linking bioenergetic function of mitochondria to tissue-specific molecular fingerprints[J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM,2019,317(2):E374-E387.
APA Kappler, Lisa.,Hoene, Miriam.,Hu, Chunxiu.,von Toerne, Christine.,Li, Jia.,...&Lehmann, Rainer.(2019).Linking bioenergetic function of mitochondria to tissue-specific molecular fingerprints.AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM,317(2),E374-E387.
MLA Kappler, Lisa,et al."Linking bioenergetic function of mitochondria to tissue-specific molecular fingerprints".AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 317.2(2019):E374-E387.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Kappler, Lisa]'s Articles
[Hoene, Miriam]'s Articles
[Hu, Chunxiu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Kappler, Lisa]'s Articles
[Hoene, Miriam]'s Articles
[Hu, Chunxiu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Kappler, Lisa]'s Articles
[Hoene, Miriam]'s Articles
[Hu, Chunxiu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.