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Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones
Chen, Yuanbo1,2; Jiang, Bo1; Hu, Yechen1,2; Deng, Nan1,3; Zhao, Baofeng1; Li, Xiao1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
Corresponding AuthorZhang, Lihua(lihuazhang@dicp.ac.cn)
KeywordEnrichment Graphene oxide Histones Magnetic nanoparticles
Source PublicationELECTROPHORESIS
2019-08-01
ISSN0173-0835
DOI10.1002/elps.201900061
Volume40Issue:16-17Pages:2135-2141
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordIN-VITRO SELECTION ; ENRICHMENT ; IMMOBILIZATION ; COMPOSITES ; EVOLUTION
AbstractThe binding coverage of aptamer was an important restricted factor for aptamer-based affinity enrichment strategy for capturing target molecules. Herein, we designed and prepared aptamer functionalized graphene oxide based nanocomposites (GO/NH2-NTA/Fe3O4/PEI/Au), and the coverage density of aptamer was high to 33.1 nmol/mg. The high aptamer coverage density was contributed to the large surface area of graphene oxide. The successive modification of N alpha,N alpha-Bis(carboxymethyl)-L-lysine, magnetic nanoparticles, polyethylenimine, and Au nanoparticles ensured the histone purification with fast speed and high purity. Histones could be captured rapidly and specifically from nucleoproteins by our aptamer based purification strategy, while traditional acid-extraction could not specifically enrich histones. Compared with traditional acid-extraction method, rapid and efficient discovery of histones and their post-translational modifications, such as several kinds of methylation at H3.1K9 and H3.1K27, were achieved confidently. It demonstrated that our aptamer functionalized magnetic graphene oxide nanocomposites have a great potential for histone analysis.
Language英语
WOS IDWOS:000478750500015
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/173994
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang, Lihua
Affiliation1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Zhengzhou Tobacco Res Inst CNTC, Zhengzhou, Henan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Yuanbo,Jiang, Bo,Hu, Yechen,et al. Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones[J]. ELECTROPHORESIS,2019,40(16-17):2135-2141.
APA Chen, Yuanbo.,Jiang, Bo.,Hu, Yechen.,Deng, Nan.,Zhao, Baofeng.,...&Zhang, Yukui.(2019).Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones.ELECTROPHORESIS,40(16-17),2135-2141.
MLA Chen, Yuanbo,et al."Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones".ELECTROPHORESIS 40.16-17(2019):2135-2141.
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