DICP OpenIR
isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy
Hu Yechen; Weng Yejing; Jiang Bo; Li Xiao; Zhang Xiaodan; Zhao Baofeng; Wu Qiong; Liang Zhen; Zhang Lihua; Zhang Yukui
Source Publicationsciencechinachemistry
2019-01-01
ISSN1674-7291
Volume62Issue:6Pages:708
Funding Project[National Key Research and Development Program of China] ; [National Natural Science Foundation of China] ; [CAS Key Project in Frontier Science] ; [DICP, Chinese Academy of Sciences]
AbstractProtein phosphorylation plays essential roles in various biological procedures. Despite the well-established enrichment strategies for O-phosphoproteomics, the intrinsic acid lability of N-P phosphoramidate bond (phosphorylation of histidine, arginine and lysine) has impaired the progress of N-phosphoproteomics. Herein, we reported a retention time difference combining dimethyl labeling (ReDD) strategy for the isolation and identification of phosphorylated lysine (pLys) peptides. By such a method, pLys peptide could be isolated under 100000-fold interference of non-phosphorylated peptides. Furthermore, ReDD strategy was applied to map pLys sites from E. coli samples, leading to the identification of 11 pLys sites, among which K26p that originating from autonomous glycyl radical cofactor was validated both in mass spectrometry and HPLC co-elution experiments. Furthermore, 112 pLys sites from 100 proteins were identified in HeLa cells. All these results demonstrate that ReDD could provide a first glimpse into Lys phosphorylation, and could be an important step toward the global perspective on protein phosphorylation.
Language英语
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/175058
Collection中国科学院大连化学物理研究所
Affiliation中国科学院大连化学物理研究所
First Author Affilication中国科学院大连化学物理研究所
Recommended Citation
GB/T 7714
Hu Yechen,Weng Yejing,Jiang Bo,et al. isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy[J]. sciencechinachemistry,2019,62(6):708.
APA Hu Yechen.,Weng Yejing.,Jiang Bo.,Li Xiao.,Zhang Xiaodan.,...&Zhang Yukui.(2019).isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy.sciencechinachemistry,62(6),708.
MLA Hu Yechen,et al."isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy".sciencechinachemistry 62.6(2019):708.
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