DICP OpenIR
Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity
Feng, Yanbin; Zhang, Yunxiu; Ding, Wei; Wu, Peichun; Cao, Xupeng; Xue, Song
Corresponding AuthorCao, Xupeng(c_x_p@dicp.ac.cn) ; Xue, Song(xuesong@dicp.ac.cn)
KeywordAcyltransferase PDAT Acyl donor Biocatalyst Enzyme Acyl acceptor
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
2019-07-01
ISSN0273-2289
DOI10.1007/s12010-019-02954-x
Volume188Issue:3Pages:824-835
Funding ProjectNational Natural Science Foundation of China[21576253] ; National Natural Science Foundation of China[21877110] ; National Natural Science Foundation of China[31470432]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS KeywordDIACYLGLYCEROL ACYLTRANSFERASE ; PHOSPHOLIPID/DIACYLGLYCEROL ACYLTRANSFERASE ; TRIACYLGLYCEROL ; ENZYME ; PLANT ; OIL ; DEGRADATION ; METABOLISM ; YEAST
AbstractTriacylglycerols are considered one of the most promising feedstocks for biofuels. Phospholipid:diacylglycerol acyltransferase (PDAT), responsible for the last step of triacylglycerol synthesis in the acyl-CoA-independent pathway, has attracted much attention by catalyzing membrane lipid transformation. However, due to lack of biochemical and enzymatic studies, PDAT has not carried forward in biocatalyst application. Here, the PDAT from Saccharomyces cerevisiae was expressed in Pichia pastoris. The purified enzymes were studied using different acyl donors and acceptors by thin layer chromatography and gas chromatography. In addition of the preferred acyl donor of PE and PC, the results identified that ScPDAT was capable of using broad acyl donors such as PA, PS, PG, MGDG, DGDG, and acyl-CoA, and ScPDAT was more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, ScPDAT preferred 1,2 to 1,3-diacylglycerol (DAG), while 12:0/12:0 DAG was identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 DAG. Additionally, ScPDAT reveals esterification activity that can utilize methanol as acyl acceptor to generate fatty acid methyl esters. The results fully expand the enzymatic selectivity of ScPDAT and provide fundamental knowledge for synthesis of triacylglycerol-derived biofuels.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS IDWOS:000471851400016
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/175941
Collection中国科学院大连化学物理研究所
Corresponding AuthorCao, Xupeng; Xue, Song
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Feng, Yanbin,Zhang, Yunxiu,Ding, Wei,et al. Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2019,188(3):824-835.
APA Feng, Yanbin,Zhang, Yunxiu,Ding, Wei,Wu, Peichun,Cao, Xupeng,&Xue, Song.(2019).Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,188(3),824-835.
MLA Feng, Yanbin,et al."Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 188.3(2019):824-835.
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