DICP OpenIR
Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry
Zhang Hua-Lin1; Guo Zhi-Mou2,3; Wang Lian-Zhi1; Jin Gao-Wa2,3; Zou Li-Hong4; Lyu Yuan-Yuan4; Ma Ming-Hui4; Yan Jing-Yu2,3; Duan Zheng-Chao1; Liang Xin-Miao2,3
Corresponding AuthorYan Jing-Yu(yanjingyu@dicp.ac.cn) ; Duan Zheng-Chao()
KeywordGanglioside Electrospray-quadrupole-time-of-flight mass spectrometry Structural analysis
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2019-06-01
ISSN0253-3820
DOI10.19756/j.issn.0253-3820.181804
Volume47Issue:6Pages:925-932
Funding ProjectNational Natural Science Foundation of China[21505131]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordLIQUID-CHROMATOGRAPHY ; IDENTIFICATION ; SEPARATION ; CERAMIDES ; COMPLEX ; CHIP
AbstractGangliosides are glycosphingolipid compounds containing sialic acid, which are natural components of vertebrate cell membranes. As a clinical drug, gangliosides are widely used in the treatment of hypoxic ischemic encephalopathy, central nervous system injury, neurodegenerative diseases and peripheral neuropathy. The structural identification of gangliosides helps to understand the composition of the drug and improve the quality of the drug. In this work, an electrospray ionization quadrupole time-of-flight mass spectrometry ( ESI-Q-TOF-MS ) method for analysis of ganglioside structure was established. The optimization of the mass spectrometry parameters were established based on testing of two gangliosides controls. The mass spectrometry conditions were established (positive mode , capillary voltage of 3500 V, fragmentor voltage of 200 V, and collision energy of 40 eV) to obtain the fragment structure of the sugar chain. The fragmentation structure of ceramide was obtained with collision energy of 80 eV. The method was applied to the structural analysis of 9 gangliosides prepared in the laboratory. The samples were categorized and classified by comparison with molecular weights of the reference substances. Detailed structure analysis was performed based on the characteristic fragments in the secondary mass spectra of the samples. The results of this study indicated that the method could be applied to the mass spectrometric identification of various gangliosides, laying a foundation for more complex ganglioside mass spectrometry analysis.
Language英语
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS IDWOS:000470983800019
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/176133
Collection中国科学院大连化学物理研究所
Corresponding AuthorYan Jing-Yu; Duan Zheng-Chao
Affiliation1.Hubei Univ Nationalities, Enshi 445000, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.DICP CMC Innovat Inst Med, Taizhou 225300, Peoples R China
4.Qilu Pharmaceut Co Ltd, Jinan 250101, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Zhang Hua-Lin,Guo Zhi-Mou,Wang Lian-Zhi,et al. Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2019,47(6):925-932.
APA Zhang Hua-Lin.,Guo Zhi-Mou.,Wang Lian-Zhi.,Jin Gao-Wa.,Zou Li-Hong.,...&Liang Xin-Miao.(2019).Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,47(6),925-932.
MLA Zhang Hua-Lin,et al."Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 47.6(2019):925-932.
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