Zheng SJ(郑思佳); Wang QQ(王晴晴); Wang XL(王晓琳); Zhao XJ(赵欣捷); Xu GW(许国旺)
Source Publicationchinesejournalofanalyticalchemistry
Funding Project[National Natural Science Foundation of China]
AbstractBladder cancer (BC) is a fatal malignancy with considerable mortality, and can cause a serious threat to human health. The successful treatment of bladder cancer relies mainly on early detection. Biomarkers are vital to early diagnosis of bladder cancer, and metabonomics play an important role in biomarkers finding. In this study, we used 69 polar metabolites to select the appropriate separation system and develop the zwitterionic hydrophilic chromatography/mass spectrometry (ZIC-HILIC/MS) method. In this method, 50 representative compounds had broad linear ranges between 2-6 orders of magnitude. Moreover the limit of detection of the method was below ng/mL levels. The analysis for six serum samples prepared in parallel showed that this method had good reproducibility, and the RSDs of more than 85% metabolites were less than 30%. Based on this method, it was found that 35 metabolites had significant differences in BC group and healthy control. After screening and validation, the combination of chenodeoxycholic acid, eicosenoic acid, GPC, dodecenoic acid and cystine was a potential biomarker to distinguish BC and normal group. These results indicated that the ZIC. HILIC/MS method could detect diverse metabolites for metabolomic analysis purpose with good reproducibility and stability.
Document Type期刊论文
First Author Affilication中国科学院大连化学物理研究所
Recommended Citation
GB/T 7714
Zheng SJ,Wang QQ,Wang XL,et al. hydrophilicinteractionliquidchromatographycoupledwithmassspectrometryforserummetabolomicsanalysisofbladdercancer[J]. chinesejournalofanalyticalchemistry,2017,45(12):1921.
APA 郑思佳,王晴晴,王晓琳,赵欣捷,&许国旺.(2017).hydrophilicinteractionliquidchromatographycoupledwithmassspectrometryforserummetabolomicsanalysisofbladdercancer.chinesejournalofanalyticalchemistry,45(12),1921.
MLA 郑思佳,et al."hydrophilicinteractionliquidchromatographycoupledwithmassspectrometryforserummetabolomicsanalysisofbladdercancer".chinesejournalofanalyticalchemistry 45.12(2017):1921.
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