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An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system
Liu, HC; Zhang, LH; Zhu, GJ; Zhang, WB; Zhang, YK
刊名ANALYTICAL CHEMISTRY
2004-11-01
76期:21页:6506-6512
收录类别SCI
文章类型Article
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]FLOW GATING INTERFACE ; ZONE-ELECTROPHORESIS ; MASS-SPECTROMETRY ; GEL-ELECTROPHORESIS ; MICROCOLUMN-LC ; CHROMATOGRAPHY ; PEPTIDES ; CZE
英文摘要The construction and evaluation of an on-column etched fused-silica porous junction for on-line coupling of capillary isoelectric focusing (CIEF) with capillary zone electrophoresis (CZE) are described. Where two separation columns were integrated on a single piece of fused-silica capillary through the etched similar to4 to 5-mm length porous junction along the capillary. The junction is easily prepared by etching a short section of the capillary wall with HF after removing the polyimide coating. The etched section becomes a porous glass membrane that allows only small ions related to the background electrolyte to pass through when high voltage is applied across the separation capillary. The primary advantages of this novel porous junction interface over previous designs (in which the interface is usually formed by fracturing the capillary followed by connecting the two capillaries with a section of microdialysis hollow fiber membrane) are no dead volume, simplicity, and ruggedness, which is particularly well suited for an on-line coupling capillary electrophoresis-based multiple dimensional separation system. The performance of the 2D CIEF-CZE system constructed by such an etched porous junction was evaluated by the analyses of protein mixtures.
语种英语
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WOS记录号WOS:000224841300051
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被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/80973
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
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Liu, HC,Zhang, LH,Zhu, GJ,et al. An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system[J]. ANALYTICAL CHEMISTRY,2004,76(21):6506-6512.
APA Liu, HC,Zhang, LH,Zhu, GJ,Zhang, WB,&Zhang, YK.(2004).An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system.ANALYTICAL CHEMISTRY,76(21),6506-6512.
MLA Liu, HC,et al."An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system".ANALYTICAL CHEMISTRY 76.21(2004):6506-6512.
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