DICP OpenIR
An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system
Liu, HC; Zhang, LH; Zhu, GJ; Zhang, WB; Zhang, YK
Source PublicationANALYTICAL CHEMISTRY
2004-11-01
Volume76Issue:21Pages:6506-6512
Indexed BySCI
SubtypeArticle
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordFLOW GATING INTERFACE ; ZONE-ELECTROPHORESIS ; MASS-SPECTROMETRY ; GEL-ELECTROPHORESIS ; MICROCOLUMN-LC ; CHROMATOGRAPHY ; PEPTIDES ; CZE
AbstractThe construction and evaluation of an on-column etched fused-silica porous junction for on-line coupling of capillary isoelectric focusing (CIEF) with capillary zone electrophoresis (CZE) are described. Where two separation columns were integrated on a single piece of fused-silica capillary through the etched similar to4 to 5-mm length porous junction along the capillary. The junction is easily prepared by etching a short section of the capillary wall with HF after removing the polyimide coating. The etched section becomes a porous glass membrane that allows only small ions related to the background electrolyte to pass through when high voltage is applied across the separation capillary. The primary advantages of this novel porous junction interface over previous designs (in which the interface is usually formed by fracturing the capillary followed by connecting the two capillaries with a section of microdialysis hollow fiber membrane) are no dead volume, simplicity, and ruggedness, which is particularly well suited for an on-line coupling capillary electrophoresis-based multiple dimensional separation system. The performance of the 2D CIEF-CZE system constructed by such an etched porous junction was evaluated by the analyses of protein mixtures.
Language英语
URL查看原文
WOS IDWOS:000224841300051
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/80973
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Liu, HC,Zhang, LH,Zhu, GJ,et al. An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system[J]. ANALYTICAL CHEMISTRY,2004,76(21):6506-6512.
APA Liu, HC,Zhang, LH,Zhu, GJ,Zhang, WB,&Zhang, YK.(2004).An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system.ANALYTICAL CHEMISTRY,76(21),6506-6512.
MLA Liu, HC,et al."An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system".ANALYTICAL CHEMISTRY 76.21(2004):6506-6512.
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