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题名: An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system
作者: Liu, HC;  Zhang, LH;  Zhu, GJ;  Zhang, WB;  Zhang, YK
刊名: ANALYTICAL CHEMISTRY
发表日期: 2004-11-01
卷: 76, 期:21, 页:6506-6512
收录类别: SCI
文章类型: Article
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The construction and evaluation of an on-column etched fused-silica porous junction for on-line coupling of capillary isoelectric focusing (CIEF) with capillary zone electrophoresis (CZE) are described. Where two separation columns were integrated on a single piece of fused-silica capillary through the etched similar to4 to 5-mm length porous junction along the capillary. The junction is easily prepared by etching a short section of the capillary wall with HF after removing the polyimide coating. The etched section becomes a porous glass membrane that allows only small ions related to the background electrolyte to pass through when high voltage is applied across the separation capillary. The primary advantages of this novel porous junction interface over previous designs (in which the interface is usually formed by fracturing the capillary followed by connecting the two capillaries with a section of microdialysis hollow fiber membrane) are no dead volume, simplicity, and ruggedness, which is particularly well suited for an on-line coupling capillary electrophoresis-based multiple dimensional separation system. The performance of the 2D CIEF-CZE system constructed by such an etched porous junction was evaluated by the analyses of protein mixtures.
关键词[WOS]: FLOW GATING INTERFACE ;  ZONE-ELECTROPHORESIS ;  MASS-SPECTROMETRY ;  GEL-ELECTROPHORESIS ;  MICROCOLUMN-LC ;  CHROMATOGRAPHY ;  PEPTIDES ;  CZE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000224841300051
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/80973
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Liu, HC,Zhang, LH,Zhu, GJ,et al. An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system[J]. ANALYTICAL CHEMISTRY,2004,76(21):6506-6512.
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