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题名: Injection by hydrostatic pressure in conjunction with electrokinetic force on a microfluidic chip
作者: Gai, HW;  Yu, LF;  Dai, ZP;  Ma, YF;  Lin, BC
关键词: hydrostatic pressure injection ;  microfluidic chip ;  miniaturization
刊名: ELECTROPHORESIS
发表日期: 2004-06-01
DOI: 10.1002/elps.200305835
卷: 25, 期:12, 页:1888-1894
收录类别: SCI
文章类型: Article
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A simple method was developed for injecting a sample on a cross-form microfluidic chip by means of hydrostatic pressure combined with electrokinetic forces. The hydrostatic pressure was generated simply by adjusting the liquid level in different reservoirs without any additional driven equipment such as a pump. Two dispensing strategies using a floating injection and a gated injection, coupled with hydrostatic pressure loading, were tested. The fluorescence observation verified the feasibility of hydrostatic pressure loading in the separation of a mixture of fluorescein sodium salt and fluorescein isothiocyanate. This method was proved to be effective in leading cells to a separation channel for single cell analysis.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  MICROCHIP ELECTROPHORESIS ;  DEVICES ;  SEPARATIONS ;  GEOMETRY ;  SYSTEM ;  BIAS ;  FLOW
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000222396000021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81089
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Missouri, Rolla, MO 65401 USA

Recommended Citation:
Gai, HW,Yu, LF,Dai, ZP,et al. Injection by hydrostatic pressure in conjunction with electrokinetic force on a microfluidic chip[J]. ELECTROPHORESIS,2004,25(12):1888-1894.
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