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题名: Recent advances in the study of biomolecular interactions by capillary electrophoresis
作者: He, XY;  Ding, YS;  Li, DZ;  Lin, BC
关键词: biomolecular interactions ;  capillary electrophoresis ;  review
刊名: ELECTROPHORESIS
发表日期: 2004-02-01
DOI: 10.1002/elps.200305727
卷: 25, 期:4-5, 页:697-711
收录类别: SCI
文章类型: Review
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Capillary electrophoresis (CE) has been abundantly used in the study of molecular interactions owing to such advantages as short analysis time, low sample size requirement, high separation efficiency, and flexible applications. The focus of this paper is to 2 review recent studies and advances (mainly from 1998 to now) in biomolecular interactions using CE. Five CE modes: zone migration CE, affinity CE, frontal analysis (FA), Hummel-Dreyer (HD) and vacancy peak (VP) are cited and compared. Quantitative aspects of the thermodynamics and kinetics of biomolecular interaction are reviewed. Several biomolecular binding systems, including protein-protein (polypeptide), protein-DNA (RNA), protein(polypeptide)-carbohydrate, protein-small molecule, DNA-small molecule, small molecule-small molecule, have been well characterized by CE. CE is shown to be a powerful tool for the determination of the binding parameters of various bioaffinity interactions.
关键词[WOS]: LASER-INDUCED FLUORESCENCE ;  HUMAN SERUM-ALBUMIN ;  PROTEIN-DRUG-BINDING ;  COMMON COLD VIRUS ;  HUMAN ALPHA(1)-ACID GLYCOPROTEIN ;  IONIZATION-MASS-SPECTROMETRY ;  HUMAN RHINOVIRUS SEROTYPE-2 ;  BOVINE CARBONIC-ANHYDRASE ;  MOBILITY SHIFT ASSAY ;  ZONE-ELECTROPHORESIS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000220046200021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81093
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Maritime Univ, Environm Sci & Engn Coll, Dalian, Peoples R China

Recommended Citation:
He, XY,Ding, YS,Li, DZ,et al. Recent advances in the study of biomolecular interactions by capillary electrophoresis[J]. ELECTROPHORESIS,2004,25(4-5):697-711.
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