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Recent advances in the study of biomolecular interactions by capillary electrophoresis
He, XY; Ding, YS; Li, DZ; Lin, BC
KeywordBiomolecular Interactions Capillary Electrophoresis Review
Source PublicationELECTROPHORESIS
2004-02-01
DOI10.1002/elps.200305727
Volume25Issue:4-5Pages:697-711
Indexed BySCI
SubtypeReview
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordLASER-INDUCED FLUORESCENCE ; HUMAN SERUM-ALBUMIN ; PROTEIN-DRUG-BINDING ; COMMON COLD VIRUS ; HUMAN ALPHA(1)-ACID GLYCOPROTEIN ; IONIZATION-MASS-SPECTROMETRY ; HUMAN RHINOVIRUS SEROTYPE-2 ; BOVINE CARBONIC-ANHYDRASE ; MOBILITY SHIFT ASSAY ; ZONE-ELECTROPHORESIS
AbstractCapillary electrophoresis (CE) has been abundantly used in the study of molecular interactions owing to such advantages as short analysis time, low sample size requirement, high separation efficiency, and flexible applications. The focus of this paper is to 2 review recent studies and advances (mainly from 1998 to now) in biomolecular interactions using CE. Five CE modes: zone migration CE, affinity CE, frontal analysis (FA), Hummel-Dreyer (HD) and vacancy peak (VP) are cited and compared. Quantitative aspects of the thermodynamics and kinetics of biomolecular interaction are reviewed. Several biomolecular binding systems, including protein-protein (polypeptide), protein-DNA (RNA), protein(polypeptide)-carbohydrate, protein-small molecule, DNA-small molecule, small molecule-small molecule, have been well characterized by CE. CE is shown to be a powerful tool for the determination of the binding parameters of various bioaffinity interactions.
Language英语
URL查看原文
WOS IDWOS:000220046200021
Citation statistics
Cited Times:94[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81093
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Maritime Univ, Environm Sci & Engn Coll, Dalian, Peoples R China
Recommended Citation
GB/T 7714
He, XY,Ding, YS,Li, DZ,et al. Recent advances in the study of biomolecular interactions by capillary electrophoresis[J]. ELECTROPHORESIS,2004,25(4-5):697-711.
APA He, XY,Ding, YS,Li, DZ,&Lin, BC.(2004).Recent advances in the study of biomolecular interactions by capillary electrophoresis.ELECTROPHORESIS,25(4-5),697-711.
MLA He, XY,et al."Recent advances in the study of biomolecular interactions by capillary electrophoresis".ELECTROPHORESIS 25.4-5(2004):697-711.
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