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题名: Determination of SARS-coronavirus by a microfluidic chip system
作者: Zhou, ZM;  Liu, DY;  Zhong, RT;  Dai, ZP;  Wu, DP;  Wang, H;  Du, YG;  Xia, ZN;  Zhang, LP;  Mei, XD;  Lin, BC
关键词: microfluidic chip system ;  miniaturization ;  multiplex reverse transcription-polymerase ;  chain reaction ;  on-line chip polymerase chain reaction ;  severe acute respiratory syndrome
刊名: ELECTROPHORESIS
发表日期: 2004-09-01
DOI: 10.1002/elps.200305965
卷: 25, 期:17, 页:3032-3039
收录类别: SCI
文章类型: Article
项目归属: 1807,1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS-Cov). The system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS-CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT-PCR on SARS-CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home-made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS-CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT-PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.
关键词[WOS]: CLINICAL-APPLICATION ;  MICROCHIP PCR ;  RAPID PCR ;  DNA ;  ELECTROPHORESIS ;  INSTRUMENT ;  ASSAY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000223938700019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/81099
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Harvard Univ, Sch Med, Dana Fabre Canc Res Inst, Boston, MA 02115 USA
3.Wyeth Pharmaceut Inc, Genom Dept, Cambridge, MA USA

Recommended Citation:
Zhou, ZM,Liu, DY,Zhong, RT,et al. Determination of SARS-coronavirus by a microfluidic chip system[J]. ELECTROPHORESIS,2004,25(17):3032-3039.
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