DICP OpenIR
Determination of SARS-coronavirus by a microfluidic chip system
Zhou, ZM; Liu, DY; Zhong, RT; Dai, ZP; Wu, DP; Wang, H; Du, YG; Xia, ZN; Zhang, LP; Mei, XD; Lin, BC
KeywordMicrofluidic Chip System Miniaturization Multiplex Reverse Transcription-polymerase Chain Reaction On-line Chip Polymerase Chain Reaction Severe Acute Respiratory Syndrome
Source PublicationELECTROPHORESIS
2004-09-01
DOI10.1002/elps.200305965
Volume25Issue:17Pages:3032-3039
Indexed BySCI
SubtypeArticle
Funding Project1807,1805
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCLINICAL-APPLICATION ; MICROCHIP PCR ; RAPID PCR ; DNA ; ELECTROPHORESIS ; INSTRUMENT ; ASSAY
AbstractWe have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS-Cov). The system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS-CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT-PCR on SARS-CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home-made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS-CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT-PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.
Language英语
URL查看原文
WOS IDWOS:000223938700019
Citation statistics
Cited Times:81[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/81099
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Harvard Univ, Sch Med, Dana Fabre Canc Res Inst, Boston, MA 02115 USA
3.Wyeth Pharmaceut Inc, Genom Dept, Cambridge, MA USA
Recommended Citation
GB/T 7714
Zhou, ZM,Liu, DY,Zhong, RT,et al. Determination of SARS-coronavirus by a microfluidic chip system[J]. ELECTROPHORESIS,2004,25(17):3032-3039.
APA Zhou, ZM.,Liu, DY.,Zhong, RT.,Dai, ZP.,Wu, DP.,...&Lin, BC.(2004).Determination of SARS-coronavirus by a microfluidic chip system.ELECTROPHORESIS,25(17),3032-3039.
MLA Zhou, ZM,et al."Determination of SARS-coronavirus by a microfluidic chip system".ELECTROPHORESIS 25.17(2004):3032-3039.
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