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Determination of SARS-coronavirus by a microfluidic chip system
Zhou, ZM; Liu, DY; Zhong, RT; Dai, ZP; Wu, DP; Wang, H; Du, YG; Xia, ZN; Zhang, LP; Mei, XD; Lin, BC
关键词Microfluidic Chip System Miniaturization Multiplex Reverse Transcription-polymerase Chain Reaction On-line Chip Polymerase Chain Reaction Severe Acute Respiratory Syndrome
刊名ELECTROPHORESIS
2004-09-01
DOI10.1002/elps.200305965
25期:17页:3032-3039
收录类别SCI
文章类型Article
项目归属1807,1805
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CLINICAL-APPLICATION ; MICROCHIP PCR ; RAPID PCR ; DNA ; ELECTROPHORESIS ; INSTRUMENT ; ASSAY
英文摘要We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS-Cov). The system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS-CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT-PCR on SARS-CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home-made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS-CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT-PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.
语种英语
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WOS记录号WOS:000223938700019
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被引频次:81[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/81099
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Harvard Univ, Sch Med, Dana Fabre Canc Res Inst, Boston, MA 02115 USA
3.Wyeth Pharmaceut Inc, Genom Dept, Cambridge, MA USA
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Zhou, ZM,Liu, DY,Zhong, RT,et al. Determination of SARS-coronavirus by a microfluidic chip system[J]. ELECTROPHORESIS,2004,25(17):3032-3039.
APA Zhou, ZM.,Liu, DY.,Zhong, RT.,Dai, ZP.,Wu, DP.,...&Lin, BC.(2004).Determination of SARS-coronavirus by a microfluidic chip system.ELECTROPHORESIS,25(17),3032-3039.
MLA Zhou, ZM,et al."Determination of SARS-coronavirus by a microfluidic chip system".ELECTROPHORESIS 25.17(2004):3032-3039.
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